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Identifying the most suitable endogenous control for determining gene expression in hearts from organ donors
BACKGROUND: Quantitative real-time reverse transcription PCR (qRT-PCR) is a useful tool for assessing gene expression in different tissues, but the choice of adequate controls is critical to normalise the results, thereby avoiding differences and maximizing sensitivity and accuracy. So far, many gen...
Autores principales: | Pérez, Silvia, Royo, Luis J, Astudillo, Aurora, Escudero, Dolores, Álvarez, Francisco, Rodríguez, Aida, Gómez, Enrique, Otero, Jesús |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234425/ https://www.ncbi.nlm.nih.gov/pubmed/18096027 http://dx.doi.org/10.1186/1471-2199-8-114 |
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