STUDIES ON PITUITARY LACTOGENIC HORMONE : I. ELECTROPHORETIC BEHAVIOR

The Tiselius technique of electrophoresis has been used to study the homogeneity and mobility of pituitary lactogenic hormone. The lactogenic preparation employed by us shows only one sharp band in schlieren photographs, indicating a high degree of homogeneity. From the determination of the mobility...

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Detalles Bibliográficos
Autores principales: Li, Choh Hao, Lyons, William R., Evans, Herbert M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1940
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2237935/
https://www.ncbi.nlm.nih.gov/pubmed/19873166
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author Li, Choh Hao
Lyons, William R.
Evans, Herbert M.
author_facet Li, Choh Hao
Lyons, William R.
Evans, Herbert M.
author_sort Li, Choh Hao
collection PubMed
description The Tiselius technique of electrophoresis has been used to study the homogeneity and mobility of pituitary lactogenic hormone. The lactogenic preparation employed by us shows only one sharp band in schlieren photographs, indicating a high degree of homogeneity. From the determination of the mobility of the hormone at different hydrogen ion concentrations in acetate and phosphate buffer of ionic strength 0.055, the isoelectric point was found to be 5.70 and the See PDF for Equation value 4.5 x 10(–5). The difference in the mobility reported for White's crystalline preparation and that found with our high potency lactogenic preparation is discussed.
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spelling pubmed-22379352008-04-23 STUDIES ON PITUITARY LACTOGENIC HORMONE : I. ELECTROPHORETIC BEHAVIOR Li, Choh Hao Lyons, William R. Evans, Herbert M. J Gen Physiol Article The Tiselius technique of electrophoresis has been used to study the homogeneity and mobility of pituitary lactogenic hormone. The lactogenic preparation employed by us shows only one sharp band in schlieren photographs, indicating a high degree of homogeneity. From the determination of the mobility of the hormone at different hydrogen ion concentrations in acetate and phosphate buffer of ionic strength 0.055, the isoelectric point was found to be 5.70 and the See PDF for Equation value 4.5 x 10(–5). The difference in the mobility reported for White's crystalline preparation and that found with our high potency lactogenic preparation is discussed. The Rockefeller University Press 1940-03-20 /pmc/articles/PMC2237935/ /pubmed/19873166 Text en Copyright © Copyright, 1940, The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Li, Choh Hao
Lyons, William R.
Evans, Herbert M.
STUDIES ON PITUITARY LACTOGENIC HORMONE : I. ELECTROPHORETIC BEHAVIOR
title STUDIES ON PITUITARY LACTOGENIC HORMONE : I. ELECTROPHORETIC BEHAVIOR
title_full STUDIES ON PITUITARY LACTOGENIC HORMONE : I. ELECTROPHORETIC BEHAVIOR
title_fullStr STUDIES ON PITUITARY LACTOGENIC HORMONE : I. ELECTROPHORETIC BEHAVIOR
title_full_unstemmed STUDIES ON PITUITARY LACTOGENIC HORMONE : I. ELECTROPHORETIC BEHAVIOR
title_short STUDIES ON PITUITARY LACTOGENIC HORMONE : I. ELECTROPHORETIC BEHAVIOR
title_sort studies on pituitary lactogenic hormone : i. electrophoretic behavior
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2237935/
https://www.ncbi.nlm.nih.gov/pubmed/19873166
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AT evansherbertm studiesonpituitarylactogenichormoneielectrophoreticbehavior

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