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SOLUBILITY STUDIES ON PURIFIED TOBACCO MOSAIC VIRUS
Different samples of purified tobacco mosaic virus show a relatively wide variation in solubility in ammonium sulfate solution. This variation and the type of solubility curve obtained in the presence of varying amounts of solid phase show that the purified virus whether isolated by mild treatment w...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1940
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2237958/ https://www.ncbi.nlm.nih.gov/pubmed/19873187 |
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author | Loring, Hubert S. |
author_facet | Loring, Hubert S. |
author_sort | Loring, Hubert S. |
collection | PubMed |
description | Different samples of purified tobacco mosaic virus show a relatively wide variation in solubility in ammonium sulfate solution. This variation and the type of solubility curve obtained in the presence of varying amounts of solid phase show that the purified virus whether isolated by mild treatment with ammonium sulfate or by ultracentrifugation is not a homogeneous chemical substance but contains more soluble and less soluble virus fractions of comparable specific activities. Long contact with strong ammonium sulfate solutions or 0.1 M phosphate buffer results in a decrease in solubility. The variation in the solubility of samples isolated from different plants by the same method seems to depend in part on the length of time the plants are inoculated before they are cut, and probably also on the conditions under which they are grown. Virus preparations isolated from plants of different genera grown under the same conditions and inoculated at the same time, however, behaved like identical substances in solubility experiments. |
format | Text |
id | pubmed-2237958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1940 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22379582008-04-23 SOLUBILITY STUDIES ON PURIFIED TOBACCO MOSAIC VIRUS Loring, Hubert S. J Gen Physiol Article Different samples of purified tobacco mosaic virus show a relatively wide variation in solubility in ammonium sulfate solution. This variation and the type of solubility curve obtained in the presence of varying amounts of solid phase show that the purified virus whether isolated by mild treatment with ammonium sulfate or by ultracentrifugation is not a homogeneous chemical substance but contains more soluble and less soluble virus fractions of comparable specific activities. Long contact with strong ammonium sulfate solutions or 0.1 M phosphate buffer results in a decrease in solubility. The variation in the solubility of samples isolated from different plants by the same method seems to depend in part on the length of time the plants are inoculated before they are cut, and probably also on the conditions under which they are grown. Virus preparations isolated from plants of different genera grown under the same conditions and inoculated at the same time, however, behaved like identical substances in solubility experiments. The Rockefeller University Press 1940-07-20 /pmc/articles/PMC2237958/ /pubmed/19873187 Text en Copyright © Copyright, 1940, The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Loring, Hubert S. SOLUBILITY STUDIES ON PURIFIED TOBACCO MOSAIC VIRUS |
title | SOLUBILITY STUDIES ON PURIFIED TOBACCO MOSAIC VIRUS |
title_full | SOLUBILITY STUDIES ON PURIFIED TOBACCO MOSAIC VIRUS |
title_fullStr | SOLUBILITY STUDIES ON PURIFIED TOBACCO MOSAIC VIRUS |
title_full_unstemmed | SOLUBILITY STUDIES ON PURIFIED TOBACCO MOSAIC VIRUS |
title_short | SOLUBILITY STUDIES ON PURIFIED TOBACCO MOSAIC VIRUS |
title_sort | solubility studies on purified tobacco mosaic virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2237958/ https://www.ncbi.nlm.nih.gov/pubmed/19873187 |
work_keys_str_mv | AT loringhuberts solubilitystudiesonpurifiedtobaccomosaicvirus |