THE PHOTODYNAMIC INACTIVATION OF PHAGE PRECURSOR BY METHYLENE BLUE

Methylene blue added to suspensions of activated staphylococci in amounts sufficient to furnish 1 x 10(5) molecules of dye/bacterium inactivates the phage precursor content of the cells without causing cell death when the mixtures are exposed to strong light of 4000–8000 Å. There is a lag phase of a...

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Detalles Bibliográficos
Autores principales: Krueger, A. P., Scribner, E. J., Mecracken, T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1940
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2237966/
https://www.ncbi.nlm.nih.gov/pubmed/19873185
Descripción
Sumario:Methylene blue added to suspensions of activated staphylococci in amounts sufficient to furnish 1 x 10(5) molecules of dye/bacterium inactivates the phage precursor content of the cells without causing cell death when the mixtures are exposed to strong light of 4000–8000 Å. There is a lag phase of approximately 15 minutes in the photodynamic inactivation of phage precursor by methylene blue. This delay seems to be due to a primary reaction between the cell and methylene blue after the completion of which exposure to light brings about the inactivation of precursor quite promptly.

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