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A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL
A simple diffusion apparatus has been described in which a layer of solution is allowed to diffuse upward into a layer of solvent. Accurate sampling is performed at various heights and the concentration of the samples is determined. The method has been illustrated with a determination of the diffusi...
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1941
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2237980/ https://www.ncbi.nlm.nih.gov/pubmed/19873229 |
| Sumario: | A simple diffusion apparatus has been described in which a layer of solution is allowed to diffuse upward into a layer of solvent. Accurate sampling is performed at various heights and the concentration of the samples is determined. The method has been illustrated with a determination of the diffusion constant of crystalline catalase, which was found to be 3.1 x 10(–7) cm.(2)/sec. at 4°C. The method should be especially suited to the study of biological substances endowed with specific activity and which cannot be obtained in pure solution. |
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