A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL

A simple diffusion apparatus has been described in which a layer of solution is allowed to diffuse upward into a layer of solvent. Accurate sampling is performed at various heights and the concentration of the samples is determined. The method has been illustrated with a determination of the diffusi...

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Detalles Bibliográficos
Autor principal: Bourdillon, Jaques
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1941
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2237980/
https://www.ncbi.nlm.nih.gov/pubmed/19873229
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author Bourdillon, Jaques
author_facet Bourdillon, Jaques
author_sort Bourdillon, Jaques
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description A simple diffusion apparatus has been described in which a layer of solution is allowed to diffuse upward into a layer of solvent. Accurate sampling is performed at various heights and the concentration of the samples is determined. The method has been illustrated with a determination of the diffusion constant of crystalline catalase, which was found to be 3.1 x 10(–7) cm.(2)/sec. at 4°C. The method should be especially suited to the study of biological substances endowed with specific activity and which cannot be obtained in pure solution.
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spelling pubmed-22379802008-04-23 A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL Bourdillon, Jaques J Gen Physiol Article A simple diffusion apparatus has been described in which a layer of solution is allowed to diffuse upward into a layer of solvent. Accurate sampling is performed at various heights and the concentration of the samples is determined. The method has been illustrated with a determination of the diffusion constant of crystalline catalase, which was found to be 3.1 x 10(–7) cm.(2)/sec. at 4°C. The method should be especially suited to the study of biological substances endowed with specific activity and which cannot be obtained in pure solution. The Rockefeller University Press 1941-03-20 /pmc/articles/PMC2237980/ /pubmed/19873229 Text en Copyright © Copyright, 1941, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Bourdillon, Jaques
A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL
title A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL
title_full A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL
title_fullStr A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL
title_full_unstemmed A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL
title_short A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL
title_sort new method for the study of diffusion of biologically active material
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2237980/
https://www.ncbi.nlm.nih.gov/pubmed/19873229
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