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SULFHYDRYL GROUPS OF EGG ALBUMIN IN DIFFERENT DENATURING AGENTS

1. The reaction between ferricyanide and egg albumin in solutions of urea, guanidine hydrochloride, and Duponol has been investigated. 2. In neutral medium ferricyanide oxidizes all the SH groups of egg albumin that give a color reaction with nitroprusside. In neutral medium ferricyanide appears to...

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Autor principal: Mirsky, A. E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1941
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2238003/
https://www.ncbi.nlm.nih.gov/pubmed/19873247
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author Mirsky, A. E.
author_facet Mirsky, A. E.
author_sort Mirsky, A. E.
collection PubMed
description 1. The reaction between ferricyanide and egg albumin in solutions of urea, guanidine hydrochloride, and Duponol has been investigated. 2. In neutral medium ferricyanide oxidizes all the SH groups of egg albumin that give a color reaction with nitroprusside. In neutral medium ferricyanide appears to react only with the SH groups of egg albumin. The quantity of ferrocyanide formed can accordingly be considered the equivalent of the number of SH groups in egg albumin detectable with nitroprusside. 3. In solutions of urea, guanidine hydrochloride, and Duponol sufficiently concentrated so that all the egg albumin present is denatured, the same number of SH groups are found—equivalent to a cysteine content of 0.96 per cent. 4. In denaturation of egg albumin loss of solubility (solubility not in presence of the denaturing agent, but solubility examined in water at the isoelectric point) and appearance of reactive SH groups are integral parts of the same process. As denaturation proceeds in urea, SH groups are liberated only in the egg albumin with altered solubility and in this albumin the maximum number of SH groups is liberated. In a molecule of egg albumin either all of its SH groups that give a test with nitroprusside are liberated or none of them are.
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spelling pubmed-22380032008-04-23 SULFHYDRYL GROUPS OF EGG ALBUMIN IN DIFFERENT DENATURING AGENTS Mirsky, A. E. J Gen Physiol Article 1. The reaction between ferricyanide and egg albumin in solutions of urea, guanidine hydrochloride, and Duponol has been investigated. 2. In neutral medium ferricyanide oxidizes all the SH groups of egg albumin that give a color reaction with nitroprusside. In neutral medium ferricyanide appears to react only with the SH groups of egg albumin. The quantity of ferrocyanide formed can accordingly be considered the equivalent of the number of SH groups in egg albumin detectable with nitroprusside. 3. In solutions of urea, guanidine hydrochloride, and Duponol sufficiently concentrated so that all the egg albumin present is denatured, the same number of SH groups are found—equivalent to a cysteine content of 0.96 per cent. 4. In denaturation of egg albumin loss of solubility (solubility not in presence of the denaturing agent, but solubility examined in water at the isoelectric point) and appearance of reactive SH groups are integral parts of the same process. As denaturation proceeds in urea, SH groups are liberated only in the egg albumin with altered solubility and in this albumin the maximum number of SH groups is liberated. In a molecule of egg albumin either all of its SH groups that give a test with nitroprusside are liberated or none of them are. The Rockefeller University Press 1941-07-20 /pmc/articles/PMC2238003/ /pubmed/19873247 Text en Copyright © Copyright, 1941, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Mirsky, A. E.
SULFHYDRYL GROUPS OF EGG ALBUMIN IN DIFFERENT DENATURING AGENTS
title SULFHYDRYL GROUPS OF EGG ALBUMIN IN DIFFERENT DENATURING AGENTS
title_full SULFHYDRYL GROUPS OF EGG ALBUMIN IN DIFFERENT DENATURING AGENTS
title_fullStr SULFHYDRYL GROUPS OF EGG ALBUMIN IN DIFFERENT DENATURING AGENTS
title_full_unstemmed SULFHYDRYL GROUPS OF EGG ALBUMIN IN DIFFERENT DENATURING AGENTS
title_short SULFHYDRYL GROUPS OF EGG ALBUMIN IN DIFFERENT DENATURING AGENTS
title_sort sulfhydryl groups of egg albumin in different denaturing agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2238003/
https://www.ncbi.nlm.nih.gov/pubmed/19873247
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