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Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower

BACKGROUND: Sunflower downy mildew is a major disease caused by the obligatory biotrophic oomycete Plasmopara halstedii. Little is known about the molecular mechanisms underlying its pathogenicity. In this study we used a genomics approach to gain a first insight into the transcriptome of P. halsted...

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Autores principales: Bouzidi, Mohamed Fouad, Parlange, Francis, Nicolas, Paul, Mouzeyar, Said
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242796/
https://www.ncbi.nlm.nih.gov/pubmed/18062809
http://dx.doi.org/10.1186/1471-2180-7-110
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author Bouzidi, Mohamed Fouad
Parlange, Francis
Nicolas, Paul
Mouzeyar, Said
author_facet Bouzidi, Mohamed Fouad
Parlange, Francis
Nicolas, Paul
Mouzeyar, Said
author_sort Bouzidi, Mohamed Fouad
collection PubMed
description BACKGROUND: Sunflower downy mildew is a major disease caused by the obligatory biotrophic oomycete Plasmopara halstedii. Little is known about the molecular mechanisms underlying its pathogenicity. In this study we used a genomics approach to gain a first insight into the transcriptome of P. halstedii. RESULTS: To identify genes from the obligatory biotrophic oomycete Plasmopara halstedii that are expressed during infection in sunflower (Helianthus annuus L.) we employed the suppression subtraction hybridization (SSH) method from sunflower seedlings infected by P. halstedii. Using this method and random sequencing of clones, a total of 602 expressed sequence tags (ESTs) corresponding to 230 unique sequence sets were identified. To determine the origin of the unisequences, PCR primers were designed to amplify these gene fragments from genomic DNA isolated either from P. halstedii sporangia or from Helianthus annuus. Only 145 nonredundant ESTs which correspond to a total of 373 ESTs (67.7%) proved to be derived from P. halstedii genes and that are expressed during infection in sunflower. A set of 87 nonredundant sequences were identified as showing matches to sequences deposited in public databases. Nevertheless, about 7% of the ESTs seem to be unique to P. halstedii without any homolog in any public database. CONCLUSION: A summary of the assignment of nonredundant ESTs to functional categories as well as their relative abundance is listed and discussed. Annotation of the ESTs revealed a number of genes that could function in virulence. We provide a first glimpse into the gene content of P. halstedii. These resources should accelerate research on this important pathogen.
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spelling pubmed-22427962008-02-14 Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower Bouzidi, Mohamed Fouad Parlange, Francis Nicolas, Paul Mouzeyar, Said BMC Microbiol Research Article BACKGROUND: Sunflower downy mildew is a major disease caused by the obligatory biotrophic oomycete Plasmopara halstedii. Little is known about the molecular mechanisms underlying its pathogenicity. In this study we used a genomics approach to gain a first insight into the transcriptome of P. halstedii. RESULTS: To identify genes from the obligatory biotrophic oomycete Plasmopara halstedii that are expressed during infection in sunflower (Helianthus annuus L.) we employed the suppression subtraction hybridization (SSH) method from sunflower seedlings infected by P. halstedii. Using this method and random sequencing of clones, a total of 602 expressed sequence tags (ESTs) corresponding to 230 unique sequence sets were identified. To determine the origin of the unisequences, PCR primers were designed to amplify these gene fragments from genomic DNA isolated either from P. halstedii sporangia or from Helianthus annuus. Only 145 nonredundant ESTs which correspond to a total of 373 ESTs (67.7%) proved to be derived from P. halstedii genes and that are expressed during infection in sunflower. A set of 87 nonredundant sequences were identified as showing matches to sequences deposited in public databases. Nevertheless, about 7% of the ESTs seem to be unique to P. halstedii without any homolog in any public database. CONCLUSION: A summary of the assignment of nonredundant ESTs to functional categories as well as their relative abundance is listed and discussed. Annotation of the ESTs revealed a number of genes that could function in virulence. We provide a first glimpse into the gene content of P. halstedii. These resources should accelerate research on this important pathogen. BioMed Central 2007-12-06 /pmc/articles/PMC2242796/ /pubmed/18062809 http://dx.doi.org/10.1186/1471-2180-7-110 Text en Copyright © 2007 Bouzidi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bouzidi, Mohamed Fouad
Parlange, Francis
Nicolas, Paul
Mouzeyar, Said
Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower
title Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower
title_full Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower
title_fullStr Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower
title_full_unstemmed Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower
title_short Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower
title_sort expressed sequence tags from the oomycete plasmopara halstedii, an obligate parasite of the sunflower
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242796/
https://www.ncbi.nlm.nih.gov/pubmed/18062809
http://dx.doi.org/10.1186/1471-2180-7-110
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