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Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower
BACKGROUND: Sunflower downy mildew is a major disease caused by the obligatory biotrophic oomycete Plasmopara halstedii. Little is known about the molecular mechanisms underlying its pathogenicity. In this study we used a genomics approach to gain a first insight into the transcriptome of P. halsted...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242796/ https://www.ncbi.nlm.nih.gov/pubmed/18062809 http://dx.doi.org/10.1186/1471-2180-7-110 |
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author | Bouzidi, Mohamed Fouad Parlange, Francis Nicolas, Paul Mouzeyar, Said |
author_facet | Bouzidi, Mohamed Fouad Parlange, Francis Nicolas, Paul Mouzeyar, Said |
author_sort | Bouzidi, Mohamed Fouad |
collection | PubMed |
description | BACKGROUND: Sunflower downy mildew is a major disease caused by the obligatory biotrophic oomycete Plasmopara halstedii. Little is known about the molecular mechanisms underlying its pathogenicity. In this study we used a genomics approach to gain a first insight into the transcriptome of P. halstedii. RESULTS: To identify genes from the obligatory biotrophic oomycete Plasmopara halstedii that are expressed during infection in sunflower (Helianthus annuus L.) we employed the suppression subtraction hybridization (SSH) method from sunflower seedlings infected by P. halstedii. Using this method and random sequencing of clones, a total of 602 expressed sequence tags (ESTs) corresponding to 230 unique sequence sets were identified. To determine the origin of the unisequences, PCR primers were designed to amplify these gene fragments from genomic DNA isolated either from P. halstedii sporangia or from Helianthus annuus. Only 145 nonredundant ESTs which correspond to a total of 373 ESTs (67.7%) proved to be derived from P. halstedii genes and that are expressed during infection in sunflower. A set of 87 nonredundant sequences were identified as showing matches to sequences deposited in public databases. Nevertheless, about 7% of the ESTs seem to be unique to P. halstedii without any homolog in any public database. CONCLUSION: A summary of the assignment of nonredundant ESTs to functional categories as well as their relative abundance is listed and discussed. Annotation of the ESTs revealed a number of genes that could function in virulence. We provide a first glimpse into the gene content of P. halstedii. These resources should accelerate research on this important pathogen. |
format | Text |
id | pubmed-2242796 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22427962008-02-14 Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower Bouzidi, Mohamed Fouad Parlange, Francis Nicolas, Paul Mouzeyar, Said BMC Microbiol Research Article BACKGROUND: Sunflower downy mildew is a major disease caused by the obligatory biotrophic oomycete Plasmopara halstedii. Little is known about the molecular mechanisms underlying its pathogenicity. In this study we used a genomics approach to gain a first insight into the transcriptome of P. halstedii. RESULTS: To identify genes from the obligatory biotrophic oomycete Plasmopara halstedii that are expressed during infection in sunflower (Helianthus annuus L.) we employed the suppression subtraction hybridization (SSH) method from sunflower seedlings infected by P. halstedii. Using this method and random sequencing of clones, a total of 602 expressed sequence tags (ESTs) corresponding to 230 unique sequence sets were identified. To determine the origin of the unisequences, PCR primers were designed to amplify these gene fragments from genomic DNA isolated either from P. halstedii sporangia or from Helianthus annuus. Only 145 nonredundant ESTs which correspond to a total of 373 ESTs (67.7%) proved to be derived from P. halstedii genes and that are expressed during infection in sunflower. A set of 87 nonredundant sequences were identified as showing matches to sequences deposited in public databases. Nevertheless, about 7% of the ESTs seem to be unique to P. halstedii without any homolog in any public database. CONCLUSION: A summary of the assignment of nonredundant ESTs to functional categories as well as their relative abundance is listed and discussed. Annotation of the ESTs revealed a number of genes that could function in virulence. We provide a first glimpse into the gene content of P. halstedii. These resources should accelerate research on this important pathogen. BioMed Central 2007-12-06 /pmc/articles/PMC2242796/ /pubmed/18062809 http://dx.doi.org/10.1186/1471-2180-7-110 Text en Copyright © 2007 Bouzidi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bouzidi, Mohamed Fouad Parlange, Francis Nicolas, Paul Mouzeyar, Said Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower |
title | Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower |
title_full | Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower |
title_fullStr | Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower |
title_full_unstemmed | Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower |
title_short | Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower |
title_sort | expressed sequence tags from the oomycete plasmopara halstedii, an obligate parasite of the sunflower |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242796/ https://www.ncbi.nlm.nih.gov/pubmed/18062809 http://dx.doi.org/10.1186/1471-2180-7-110 |
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