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Effect of Intestinal Microflora on the Production of Interleukin 10 and Prostaglandin E(2) in Serum and Kupffer Cells from Germfree and Conventional Mice
To determine why germfree (GF) mice are less productivity of proinflammatory cytokines than conventional (CV) mice, we studied serum levels of interleukin 10 (IL-10) and prostaglandin E(2) (PGE(2)) in mice after treatment with lipopolyssacharide (LPS). A single injection of LPS caused an elevation o...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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the Society for Free Radical Research Japan
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2243250/ https://www.ncbi.nlm.nih.gov/pubmed/18299711 http://dx.doi.org/10.3164/jcbn.2007023 |
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author | Ikeda, Masamichi Ohira, Hideo Toyama, Yukiko Katagiri, Tikae Sakakibara, Bunsaku |
author_facet | Ikeda, Masamichi Ohira, Hideo Toyama, Yukiko Katagiri, Tikae Sakakibara, Bunsaku |
author_sort | Ikeda, Masamichi |
collection | PubMed |
description | To determine why germfree (GF) mice are less productivity of proinflammatory cytokines than conventional (CV) mice, we studied serum levels of interleukin 10 (IL-10) and prostaglandin E(2) (PGE(2)) in mice after treatment with lipopolyssacharide (LPS). A single injection of LPS caused an elevation of IL-10 in serum from GF, LPS-GF (germfree mice given drinking water containing LPS) and CV mice. The response was highest in serum from GF mice, and was lower in serum from LPS-GF mice compared with GF mice. Before LPS injection, serum PGE(2) was significantly higher in CV and LPS-GF mice than in GF ones. After LPS injection, a higher level of PGE(2) was maintained over 12 h in CV mice after LPS injection, while the LPS treatment reduced the level in LPS-GF mice and increased the level in GF mice. The levels of IL-10 in culture medium from Kupffer cells treated with LPS showed similar results to serum in GF and CV mice. These results suggest that high levels of IL-10 in serum from germfree mice may be partly responsible for the lower in vivo responsiveness of these proinflammatory cytokines to LPS in these mice, although PGE(2) was not responsible for the lower responsiveness of these inflammatory cytokines to LPS. |
format | Text |
id | pubmed-2243250 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | the Society for Free Radical Research Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-22432502008-02-25 Effect of Intestinal Microflora on the Production of Interleukin 10 and Prostaglandin E(2) in Serum and Kupffer Cells from Germfree and Conventional Mice Ikeda, Masamichi Ohira, Hideo Toyama, Yukiko Katagiri, Tikae Sakakibara, Bunsaku J Clin Biochem Nutr Original Article To determine why germfree (GF) mice are less productivity of proinflammatory cytokines than conventional (CV) mice, we studied serum levels of interleukin 10 (IL-10) and prostaglandin E(2) (PGE(2)) in mice after treatment with lipopolyssacharide (LPS). A single injection of LPS caused an elevation of IL-10 in serum from GF, LPS-GF (germfree mice given drinking water containing LPS) and CV mice. The response was highest in serum from GF mice, and was lower in serum from LPS-GF mice compared with GF mice. Before LPS injection, serum PGE(2) was significantly higher in CV and LPS-GF mice than in GF ones. After LPS injection, a higher level of PGE(2) was maintained over 12 h in CV mice after LPS injection, while the LPS treatment reduced the level in LPS-GF mice and increased the level in GF mice. The levels of IL-10 in culture medium from Kupffer cells treated with LPS showed similar results to serum in GF and CV mice. These results suggest that high levels of IL-10 in serum from germfree mice may be partly responsible for the lower in vivo responsiveness of these proinflammatory cytokines to LPS in these mice, although PGE(2) was not responsible for the lower responsiveness of these inflammatory cytokines to LPS. the Society for Free Radical Research Japan 2007-11 2007-10-30 /pmc/articles/PMC2243250/ /pubmed/18299711 http://dx.doi.org/10.3164/jcbn.2007023 Text en Copyright © 2007 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ikeda, Masamichi Ohira, Hideo Toyama, Yukiko Katagiri, Tikae Sakakibara, Bunsaku Effect of Intestinal Microflora on the Production of Interleukin 10 and Prostaglandin E(2) in Serum and Kupffer Cells from Germfree and Conventional Mice |
title | Effect of Intestinal Microflora on the Production of Interleukin 10 and Prostaglandin E(2) in Serum and Kupffer Cells from Germfree and Conventional Mice |
title_full | Effect of Intestinal Microflora on the Production of Interleukin 10 and Prostaglandin E(2) in Serum and Kupffer Cells from Germfree and Conventional Mice |
title_fullStr | Effect of Intestinal Microflora on the Production of Interleukin 10 and Prostaglandin E(2) in Serum and Kupffer Cells from Germfree and Conventional Mice |
title_full_unstemmed | Effect of Intestinal Microflora on the Production of Interleukin 10 and Prostaglandin E(2) in Serum and Kupffer Cells from Germfree and Conventional Mice |
title_short | Effect of Intestinal Microflora on the Production of Interleukin 10 and Prostaglandin E(2) in Serum and Kupffer Cells from Germfree and Conventional Mice |
title_sort | effect of intestinal microflora on the production of interleukin 10 and prostaglandin e(2) in serum and kupffer cells from germfree and conventional mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2243250/ https://www.ncbi.nlm.nih.gov/pubmed/18299711 http://dx.doi.org/10.3164/jcbn.2007023 |
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