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Discovery and characterization of a putrescine oxidase from Rhodococcus erythropolis NCIMB 11540
A gene encoding a putrescine oxidase (PuO(Rh), EC 1.4.3.10) was identified from the genome of Rhodococcus erythropolis NCIMB 11540. The gene was cloned in the pBAD vector and overexpressed at high levels in Escherichia coli. The purified enzyme was shown to be a soluble dimeric flavoprotein consisti...
Autores principales: | van Hellemond, Erik W., van Dijk, Marianne, Heuts, Dominic P. H. M., Janssen, Dick B., Fraaije, Marco W. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2243256/ https://www.ncbi.nlm.nih.gov/pubmed/18183391 http://dx.doi.org/10.1007/s00253-007-1310-4 |
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