High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array

We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the...

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Detalles Bibliográficos
Autores principales: Spurgeon, Sandra L., Jones, Robert C., Ramakrishnan, Ramesh
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2244704/
https://www.ncbi.nlm.nih.gov/pubmed/18301740
http://dx.doi.org/10.1371/journal.pone.0001662
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author Spurgeon, Sandra L.
Jones, Robert C.
Ramakrishnan, Ramesh
author_facet Spurgeon, Sandra L.
Jones, Robert C.
Ramakrishnan, Ramesh
author_sort Spurgeon, Sandra L.
collection PubMed
description We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.
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spelling pubmed-22447042008-02-27 High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array Spurgeon, Sandra L. Jones, Robert C. Ramakrishnan, Ramesh PLoS One Research Article We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays. Public Library of Science 2008-02-27 /pmc/articles/PMC2244704/ /pubmed/18301740 http://dx.doi.org/10.1371/journal.pone.0001662 Text en Spurgeon et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Spurgeon, Sandra L.
Jones, Robert C.
Ramakrishnan, Ramesh
High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array
title High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array
title_full High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array
title_fullStr High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array
title_full_unstemmed High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array
title_short High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array
title_sort high throughput gene expression measurement with real time pcr in a microfluidic dynamic array
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2244704/
https://www.ncbi.nlm.nih.gov/pubmed/18301740
http://dx.doi.org/10.1371/journal.pone.0001662
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