Direct selection and phage display of a Gram-positive secretome

Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified ind...

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Detalles Bibliográficos
Autores principales: Jankovic, Dragana, Collett, Michael A, Lubbers, Mark W, Rakonjac, Jasna
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246268/
https://www.ncbi.nlm.nih.gov/pubmed/18078523
http://dx.doi.org/10.1186/gb-2007-8-12-r266
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author Jankovic, Dragana
Collett, Michael A
Lubbers, Mark W
Rakonjac, Jasna
author_facet Jankovic, Dragana
Collett, Michael A
Lubbers, Mark W
Rakonjac, Jasna
author_sort Jankovic, Dragana
collection PubMed
description Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins.
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spelling pubmed-22462682008-02-20 Direct selection and phage display of a Gram-positive secretome Jankovic, Dragana Collett, Michael A Lubbers, Mark W Rakonjac, Jasna Genome Biol Method Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins. BioMed Central 2007 2007-12-13 /pmc/articles/PMC2246268/ /pubmed/18078523 http://dx.doi.org/10.1186/gb-2007-8-12-r266 Text en Copyright © 2007 Jankovic et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Jankovic, Dragana
Collett, Michael A
Lubbers, Mark W
Rakonjac, Jasna
Direct selection and phage display of a Gram-positive secretome
title Direct selection and phage display of a Gram-positive secretome
title_full Direct selection and phage display of a Gram-positive secretome
title_fullStr Direct selection and phage display of a Gram-positive secretome
title_full_unstemmed Direct selection and phage display of a Gram-positive secretome
title_short Direct selection and phage display of a Gram-positive secretome
title_sort direct selection and phage display of a gram-positive secretome
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246268/
https://www.ncbi.nlm.nih.gov/pubmed/18078523
http://dx.doi.org/10.1186/gb-2007-8-12-r266
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