Post-translational modifications of histones H3 and H4 associated with the histone methyltransferases Suv39h1 and G9a

Specific combinations of post-translational modifications of histones alter chromatin structure, facilitating gene transcription or silencing. Here we have investigated the 'histone code' associated with the histone methyltransferases Suv39h1 and G9a by combining double immunopurification...

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Detalles Bibliográficos
Autores principales: Robin, Philippe, Fritsch, Lauriane, Philipot, Ophélie, Svinarchuk, Fedor, Ait-Si-Ali, Slimane
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246272/
https://www.ncbi.nlm.nih.gov/pubmed/18096052
http://dx.doi.org/10.1186/gb-2007-8-12-r270
Descripción
Sumario:Specific combinations of post-translational modifications of histones alter chromatin structure, facilitating gene transcription or silencing. Here we have investigated the 'histone code' associated with the histone methyltransferases Suv39h1 and G9a by combining double immunopurification and mass spectrometry. Our results confirm the previously reported histone modifications associated with Suv39h1 and G9a. Moreover, this method allowed us to demonstrate for the first time an association of acetylated histones with the repressor proteins Suv39h1 and G9a.

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