Detection of chromosomal 7 loss in myelodysplasia using an extremely polymorphic DNA probe.

Chromosomal loss is a characteristic feature of the myelodysplastic syndromes (MDS). A method is described which detects chromosomal 7 loss in MDS by DNA analysis using a specific hypervariable region gene probe which has been cloned from a human DNA fingerprint. Loss of one of the chromosomal 7 hom...

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Autores principales: Thein, S. L., Oscier, D. G., Jeffreys, A. J., Hesketh, C., Pilkington, S., Summers, C., Fitchett, M., Wainscoat, J. S.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1988
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246429/
https://www.ncbi.nlm.nih.gov/pubmed/3358903
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author Thein, S. L.
Oscier, D. G.
Jeffreys, A. J.
Hesketh, C.
Pilkington, S.
Summers, C.
Fitchett, M.
Wainscoat, J. S.
author_facet Thein, S. L.
Oscier, D. G.
Jeffreys, A. J.
Hesketh, C.
Pilkington, S.
Summers, C.
Fitchett, M.
Wainscoat, J. S.
author_sort Thein, S. L.
collection PubMed
description Chromosomal loss is a characteristic feature of the myelodysplastic syndromes (MDS). A method is described which detects chromosomal 7 loss in MDS by DNA analysis using a specific hypervariable region gene probe which has been cloned from a human DNA fingerprint. Loss of one of the chromosomal 7 homologues was demonstrated in 10/118 MDS patients; the ten patients include all the five patients which had previously been shown to have monosomy 7 by cytogenetic analysis. This technique makes it feasible to study serial samples from large numbers of patients for loss of chromosomal material and could be readily applied to the study of other human malignancies. IMAGES:
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spelling pubmed-22464292009-09-10 Detection of chromosomal 7 loss in myelodysplasia using an extremely polymorphic DNA probe. Thein, S. L. Oscier, D. G. Jeffreys, A. J. Hesketh, C. Pilkington, S. Summers, C. Fitchett, M. Wainscoat, J. S. Br J Cancer Research Article Chromosomal loss is a characteristic feature of the myelodysplastic syndromes (MDS). A method is described which detects chromosomal 7 loss in MDS by DNA analysis using a specific hypervariable region gene probe which has been cloned from a human DNA fingerprint. Loss of one of the chromosomal 7 homologues was demonstrated in 10/118 MDS patients; the ten patients include all the five patients which had previously been shown to have monosomy 7 by cytogenetic analysis. This technique makes it feasible to study serial samples from large numbers of patients for loss of chromosomal material and could be readily applied to the study of other human malignancies. IMAGES: Nature Publishing Group 1988-02 /pmc/articles/PMC2246429/ /pubmed/3358903 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Thein, S. L.
Oscier, D. G.
Jeffreys, A. J.
Hesketh, C.
Pilkington, S.
Summers, C.
Fitchett, M.
Wainscoat, J. S.
Detection of chromosomal 7 loss in myelodysplasia using an extremely polymorphic DNA probe.
title Detection of chromosomal 7 loss in myelodysplasia using an extremely polymorphic DNA probe.
title_full Detection of chromosomal 7 loss in myelodysplasia using an extremely polymorphic DNA probe.
title_fullStr Detection of chromosomal 7 loss in myelodysplasia using an extremely polymorphic DNA probe.
title_full_unstemmed Detection of chromosomal 7 loss in myelodysplasia using an extremely polymorphic DNA probe.
title_short Detection of chromosomal 7 loss in myelodysplasia using an extremely polymorphic DNA probe.
title_sort detection of chromosomal 7 loss in myelodysplasia using an extremely polymorphic dna probe.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246429/
https://www.ncbi.nlm.nih.gov/pubmed/3358903
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