Detection of circulating antibodies against c-myc protein in cancer patient sera.

We have partially purified an archaebacterial protein of 84 kD which shares common epitopes with the human c-myc protein as shown by its cross-reactivity with a commercialized anti-human c-myc antiserum. An antiserum raised against the 84 kD protein recognizes a 60 kD protein from HL-60 nuclei. This...

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Autores principales: Ben-Mahrez, K., Thierry, D., Sorokine, I., Danna-Muller, A., Kohiyama, M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1988
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246462/
https://www.ncbi.nlm.nih.gov/pubmed/3044429
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author Ben-Mahrez, K.
Thierry, D.
Sorokine, I.
Danna-Muller, A.
Kohiyama, M.
author_facet Ben-Mahrez, K.
Thierry, D.
Sorokine, I.
Danna-Muller, A.
Kohiyama, M.
author_sort Ben-Mahrez, K.
collection PubMed
description We have partially purified an archaebacterial protein of 84 kD which shares common epitopes with the human c-myc protein as shown by its cross-reactivity with a commercialized anti-human c-myc antiserum. An antiserum raised against the 84 kD protein recognizes a 60 kD protein from HL-60 nuclei. This protein is also recognized by the anti-human c-myc antiserum. Using this archaebacterial protein as antigen for Western blot analysis, we found that the human c-myc oncogene product could be immunogenic and that it is possible, in some spontaneously occurring human tumours, to detect antibodies against the c-myc gene product in the serum of cancer patients. IMAGES:
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spelling pubmed-22464622009-09-10 Detection of circulating antibodies against c-myc protein in cancer patient sera. Ben-Mahrez, K. Thierry, D. Sorokine, I. Danna-Muller, A. Kohiyama, M. Br J Cancer Research Article We have partially purified an archaebacterial protein of 84 kD which shares common epitopes with the human c-myc protein as shown by its cross-reactivity with a commercialized anti-human c-myc antiserum. An antiserum raised against the 84 kD protein recognizes a 60 kD protein from HL-60 nuclei. This protein is also recognized by the anti-human c-myc antiserum. Using this archaebacterial protein as antigen for Western blot analysis, we found that the human c-myc oncogene product could be immunogenic and that it is possible, in some spontaneously occurring human tumours, to detect antibodies against the c-myc gene product in the serum of cancer patients. IMAGES: Nature Publishing Group 1988-06 /pmc/articles/PMC2246462/ /pubmed/3044429 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Ben-Mahrez, K.
Thierry, D.
Sorokine, I.
Danna-Muller, A.
Kohiyama, M.
Detection of circulating antibodies against c-myc protein in cancer patient sera.
title Detection of circulating antibodies against c-myc protein in cancer patient sera.
title_full Detection of circulating antibodies against c-myc protein in cancer patient sera.
title_fullStr Detection of circulating antibodies against c-myc protein in cancer patient sera.
title_full_unstemmed Detection of circulating antibodies against c-myc protein in cancer patient sera.
title_short Detection of circulating antibodies against c-myc protein in cancer patient sera.
title_sort detection of circulating antibodies against c-myc protein in cancer patient sera.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246462/
https://www.ncbi.nlm.nih.gov/pubmed/3044429
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