A tumour spheroid model for antibody-targeted therapy of micrometastases.

Human neuroblastoma cells grown as tumour spheroids were briefly incubated with a conjugate of 131I and an anti-human neuroectodermal monoclonal antibody UJ13A. Unbound 131I was removed by washing and the spheroids observed in culture conditions for up to 4 weeks. Spheroid response to irradiation wa...

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Autores principales: Walker, K. A., Murray, T., Hilditch, T. E., Wheldon, T. E., Gregor, A., Hann, I. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1988
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246487/
https://www.ncbi.nlm.nih.gov/pubmed/3166889
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author Walker, K. A.
Murray, T.
Hilditch, T. E.
Wheldon, T. E.
Gregor, A.
Hann, I. M.
author_facet Walker, K. A.
Murray, T.
Hilditch, T. E.
Wheldon, T. E.
Gregor, A.
Hann, I. M.
author_sort Walker, K. A.
collection PubMed
description Human neuroblastoma cells grown as tumour spheroids were briefly incubated with a conjugate of 131I and an anti-human neuroectodermal monoclonal antibody UJ13A. Unbound 131I was removed by washing and the spheroids observed in culture conditions for up to 4 weeks. Spheroid response to irradiation was evaluated as time to reach 10x treatment volume and proportion of spheroids sterilised. Spheroid growth was found to be affected by both the activity of 131I-UJ13A and the duration of the incubation. Na[131I], 131I-HSA, 131I labelled non-specific antibody and unlabelled antibody were found to be relatively ineffective compared to 131I-UJ13A. The tumour spheroid model has applications in the evaluation of antibodies or antibody fragments and different radionuclides which may be considered for radioimmunotherapy of micrometastases.
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spelling pubmed-22464872009-09-10 A tumour spheroid model for antibody-targeted therapy of micrometastases. Walker, K. A. Murray, T. Hilditch, T. E. Wheldon, T. E. Gregor, A. Hann, I. M. Br J Cancer Research Article Human neuroblastoma cells grown as tumour spheroids were briefly incubated with a conjugate of 131I and an anti-human neuroectodermal monoclonal antibody UJ13A. Unbound 131I was removed by washing and the spheroids observed in culture conditions for up to 4 weeks. Spheroid response to irradiation was evaluated as time to reach 10x treatment volume and proportion of spheroids sterilised. Spheroid growth was found to be affected by both the activity of 131I-UJ13A and the duration of the incubation. Na[131I], 131I-HSA, 131I labelled non-specific antibody and unlabelled antibody were found to be relatively ineffective compared to 131I-UJ13A. The tumour spheroid model has applications in the evaluation of antibodies or antibody fragments and different radionuclides which may be considered for radioimmunotherapy of micrometastases. Nature Publishing Group 1988-07 /pmc/articles/PMC2246487/ /pubmed/3166889 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Walker, K. A.
Murray, T.
Hilditch, T. E.
Wheldon, T. E.
Gregor, A.
Hann, I. M.
A tumour spheroid model for antibody-targeted therapy of micrometastases.
title A tumour spheroid model for antibody-targeted therapy of micrometastases.
title_full A tumour spheroid model for antibody-targeted therapy of micrometastases.
title_fullStr A tumour spheroid model for antibody-targeted therapy of micrometastases.
title_full_unstemmed A tumour spheroid model for antibody-targeted therapy of micrometastases.
title_short A tumour spheroid model for antibody-targeted therapy of micrometastases.
title_sort tumour spheroid model for antibody-targeted therapy of micrometastases.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246487/
https://www.ncbi.nlm.nih.gov/pubmed/3166889
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