Reactivity of monoclonal antibodies to oncoproteins with normal rat liver, carcinogen-induced tumours, and premalignant liver lesions.

Monoclonal antibodies to proteins encoded by the ras, myb, myc, erb-B, src and PDGF-2 genes were tested for reactivity with normal rat liver, livers from rats fed with 0.06% 2-acetylaminofluorene (AAF), and premalignant lesions and primary liver tumours from rats given AAF alone or a combined treatm...

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Detalles Bibliográficos
Autores principales: Embleton, M. J., Butler, P. C.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1988
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246684/
https://www.ncbi.nlm.nih.gov/pubmed/3279994
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author Embleton, M. J.
Butler, P. C.
author_facet Embleton, M. J.
Butler, P. C.
author_sort Embleton, M. J.
collection PubMed
description Monoclonal antibodies to proteins encoded by the ras, myb, myc, erb-B, src and PDGF-2 genes were tested for reactivity with normal rat liver, livers from rats fed with 0.06% 2-acetylaminofluorene (AAF), and premalignant lesions and primary liver tumours from rats given AAF alone or a combined treatment with diethylnitrosamine and AAF. Radioimmunoassays were performed with plasma membrane fractions and total soluble subcellular extracts of the tissues, and immunoperoxidase staining was carried out on frozen tissue sections. All of the antibodies were positive in radioimmunoassays, some more strongly than others, and each antibody bound equally to extracts of different kinds of tissue. Immunohistology revealed significant staining of normal liver by 5 of the 6 antibodies, and only minor qualitative differences of the staining pattern in some tumours and hyperplastic nodules. It was concluded that these antibodies were not able to discriminate sufficiently well between normal, premalignant and malignant rat liver to be of value in identifying the precursor cells of malignant tumours. IMAGES:
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spelling pubmed-22466842009-09-10 Reactivity of monoclonal antibodies to oncoproteins with normal rat liver, carcinogen-induced tumours, and premalignant liver lesions. Embleton, M. J. Butler, P. C. Br J Cancer Research Article Monoclonal antibodies to proteins encoded by the ras, myb, myc, erb-B, src and PDGF-2 genes were tested for reactivity with normal rat liver, livers from rats fed with 0.06% 2-acetylaminofluorene (AAF), and premalignant lesions and primary liver tumours from rats given AAF alone or a combined treatment with diethylnitrosamine and AAF. Radioimmunoassays were performed with plasma membrane fractions and total soluble subcellular extracts of the tissues, and immunoperoxidase staining was carried out on frozen tissue sections. All of the antibodies were positive in radioimmunoassays, some more strongly than others, and each antibody bound equally to extracts of different kinds of tissue. Immunohistology revealed significant staining of normal liver by 5 of the 6 antibodies, and only minor qualitative differences of the staining pattern in some tumours and hyperplastic nodules. It was concluded that these antibodies were not able to discriminate sufficiently well between normal, premalignant and malignant rat liver to be of value in identifying the precursor cells of malignant tumours. IMAGES: Nature Publishing Group 1988-01 /pmc/articles/PMC2246684/ /pubmed/3279994 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Embleton, M. J.
Butler, P. C.
Reactivity of monoclonal antibodies to oncoproteins with normal rat liver, carcinogen-induced tumours, and premalignant liver lesions.
title Reactivity of monoclonal antibodies to oncoproteins with normal rat liver, carcinogen-induced tumours, and premalignant liver lesions.
title_full Reactivity of monoclonal antibodies to oncoproteins with normal rat liver, carcinogen-induced tumours, and premalignant liver lesions.
title_fullStr Reactivity of monoclonal antibodies to oncoproteins with normal rat liver, carcinogen-induced tumours, and premalignant liver lesions.
title_full_unstemmed Reactivity of monoclonal antibodies to oncoproteins with normal rat liver, carcinogen-induced tumours, and premalignant liver lesions.
title_short Reactivity of monoclonal antibodies to oncoproteins with normal rat liver, carcinogen-induced tumours, and premalignant liver lesions.
title_sort reactivity of monoclonal antibodies to oncoproteins with normal rat liver, carcinogen-induced tumours, and premalignant liver lesions.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246684/
https://www.ncbi.nlm.nih.gov/pubmed/3279994
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