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Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A.

A new cell line Rat mammary (Rama) 900 was isolated from the ascitic version of the SMT-2A metastasising rat mammary tumour by stepwise adaptation of the tumour cells to tissue culture. The cells grew mainly as loosely-adherent aggregates, and were dependent during the first 18 passages in vitro on...

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Autores principales: Rudland, P. S., Dunnington, D. J., Kim, U., Gusterson, B. A., O'Hare, M. J., Monaghan, P.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246738/
https://www.ncbi.nlm.nih.gov/pubmed/2736223
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author Rudland, P. S.
Dunnington, D. J.
Kim, U.
Gusterson, B. A.
O'Hare, M. J.
Monaghan, P.
author_facet Rudland, P. S.
Dunnington, D. J.
Kim, U.
Gusterson, B. A.
O'Hare, M. J.
Monaghan, P.
author_sort Rudland, P. S.
collection PubMed
description A new cell line Rat mammary (Rama) 900 was isolated from the ascitic version of the SMT-2A metastasising rat mammary tumour by stepwise adaptation of the tumour cells to tissue culture. The cells grew mainly as loosely-adherent aggregates, and were dependent during the first 18 passages in vitro on a feeder layer of mesothelial-like cells (Rama 950) obtained from the same tumour. Subcutaneous injection of Rama 900 cells in fat pads of syngeneic Wistar Furth rats yielded anaplastic primary tumours and extensive, gross metastases including those in lungs, lymph nodes, liver and bones, similar to the parental transplantable tumour. The extent of metastatic spread from subcutaneous fat pads was increased by passage 17 in vitro for the Rama 900 cells. A similar extent of metastatic spread was achieved at earlier times by injecting the original cells with the non-tumorigenic Rama 950 cells in vivo. Subcutaneous injection of Rama 900 into thymectomised rats or MF1 nu/nu mice yielded fewer tumours, most of which regressed. No metastases occurred in the thymectomised rats and fewer metastases, mainly in lungs but not in lymph nodes, livers or bones, were seen in the nude mice. The ascitic tumours formed by intraperitoneal injection of nude mice contained both anaplastic rat cells similar to Rama 900 and mouse mesothelial-like cells similar to Rama 950. Although these anaplastic ascites cells failed to yield any tumours in syngeneic or thymectomised rats, they still produced tumours and metastases, including those in lymph nodes, in nude mice. IMAGES:
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spelling pubmed-22467382009-09-10 Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A. Rudland, P. S. Dunnington, D. J. Kim, U. Gusterson, B. A. O'Hare, M. J. Monaghan, P. Br J Cancer Research Article A new cell line Rat mammary (Rama) 900 was isolated from the ascitic version of the SMT-2A metastasising rat mammary tumour by stepwise adaptation of the tumour cells to tissue culture. The cells grew mainly as loosely-adherent aggregates, and were dependent during the first 18 passages in vitro on a feeder layer of mesothelial-like cells (Rama 950) obtained from the same tumour. Subcutaneous injection of Rama 900 cells in fat pads of syngeneic Wistar Furth rats yielded anaplastic primary tumours and extensive, gross metastases including those in lungs, lymph nodes, liver and bones, similar to the parental transplantable tumour. The extent of metastatic spread from subcutaneous fat pads was increased by passage 17 in vitro for the Rama 900 cells. A similar extent of metastatic spread was achieved at earlier times by injecting the original cells with the non-tumorigenic Rama 950 cells in vivo. Subcutaneous injection of Rama 900 into thymectomised rats or MF1 nu/nu mice yielded fewer tumours, most of which regressed. No metastases occurred in the thymectomised rats and fewer metastases, mainly in lungs but not in lymph nodes, livers or bones, were seen in the nude mice. The ascitic tumours formed by intraperitoneal injection of nude mice contained both anaplastic rat cells similar to Rama 900 and mouse mesothelial-like cells similar to Rama 950. Although these anaplastic ascites cells failed to yield any tumours in syngeneic or thymectomised rats, they still produced tumours and metastases, including those in lymph nodes, in nude mice. IMAGES: Nature Publishing Group 1989-06 /pmc/articles/PMC2246738/ /pubmed/2736223 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Rudland, P. S.
Dunnington, D. J.
Kim, U.
Gusterson, B. A.
O'Hare, M. J.
Monaghan, P.
Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A.
title Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A.
title_full Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A.
title_fullStr Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A.
title_full_unstemmed Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A.
title_short Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A.
title_sort isolation and properties of cell lines from the metastasising rat mammary tumour smt-2a.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246738/
https://www.ncbi.nlm.nih.gov/pubmed/2736223
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