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In vitro malignant progression of cells derived from Abelson murine leukaemia virus-induced thymic lymphomas.
Cell lines derived from A-MuLV induced thymic lymphomas in BALB/c and C57BL/6 mice were analysed for their in vivo and in vitro potential of growth. Despite their immunogenicity, cell lines of BALB/c origin readily grew in syngeneic recipients. On the contrary, all cell lines of C57BL/6 origin faile...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1988
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246750/ https://www.ncbi.nlm.nih.gov/pubmed/3262364 |
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author | Saggioro, D. Zamarchi, R. D'Andrea, E. Chieco-Bianchi, L. |
author_facet | Saggioro, D. Zamarchi, R. D'Andrea, E. Chieco-Bianchi, L. |
author_sort | Saggioro, D. |
collection | PubMed |
description | Cell lines derived from A-MuLV induced thymic lymphomas in BALB/c and C57BL/6 mice were analysed for their in vivo and in vitro potential of growth. Despite their immunogenicity, cell lines of BALB/c origin readily grew in syngeneic recipients. On the contrary, all cell lines of C57BL/6 origin failed to grow in immunocompetent hosts even though they were able to form tumours in immunosuppressed syngeneic mice. Among C57BL/6 lymphoma cells progression toward a more malignant phenotype was observed in TB6-3 cells, and in their derived clones, after several in vitro passages. This event was accompanied by the in vitro loss of requirement for exogenous growth factor(s) when tumorigenic TB6-3 cells were plated at high density. Moreover, culture medium from fully malignant TB-3 cells was mitogenic for mature T-lymphoma cells suggesting the involvement of an autocrine mechanism in the control of cell proliferation. Apparently, the viral oncogene (v-abl) is not directly involved in malignant progression since no differences between nontumorigenic and tumorigenic cells could be detected in A-MuLV integration patterns, v-abl specific mRNA expression, and P160gag-abl production. IMAGES: |
format | Text |
id | pubmed-2246750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1988 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-22467502009-09-10 In vitro malignant progression of cells derived from Abelson murine leukaemia virus-induced thymic lymphomas. Saggioro, D. Zamarchi, R. D'Andrea, E. Chieco-Bianchi, L. Br J Cancer Research Article Cell lines derived from A-MuLV induced thymic lymphomas in BALB/c and C57BL/6 mice were analysed for their in vivo and in vitro potential of growth. Despite their immunogenicity, cell lines of BALB/c origin readily grew in syngeneic recipients. On the contrary, all cell lines of C57BL/6 origin failed to grow in immunocompetent hosts even though they were able to form tumours in immunosuppressed syngeneic mice. Among C57BL/6 lymphoma cells progression toward a more malignant phenotype was observed in TB6-3 cells, and in their derived clones, after several in vitro passages. This event was accompanied by the in vitro loss of requirement for exogenous growth factor(s) when tumorigenic TB6-3 cells were plated at high density. Moreover, culture medium from fully malignant TB-3 cells was mitogenic for mature T-lymphoma cells suggesting the involvement of an autocrine mechanism in the control of cell proliferation. Apparently, the viral oncogene (v-abl) is not directly involved in malignant progression since no differences between nontumorigenic and tumorigenic cells could be detected in A-MuLV integration patterns, v-abl specific mRNA expression, and P160gag-abl production. IMAGES: Nature Publishing Group 1988-08 /pmc/articles/PMC2246750/ /pubmed/3262364 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Saggioro, D. Zamarchi, R. D'Andrea, E. Chieco-Bianchi, L. In vitro malignant progression of cells derived from Abelson murine leukaemia virus-induced thymic lymphomas. |
title | In vitro malignant progression of cells derived from Abelson murine leukaemia virus-induced thymic lymphomas. |
title_full | In vitro malignant progression of cells derived from Abelson murine leukaemia virus-induced thymic lymphomas. |
title_fullStr | In vitro malignant progression of cells derived from Abelson murine leukaemia virus-induced thymic lymphomas. |
title_full_unstemmed | In vitro malignant progression of cells derived from Abelson murine leukaemia virus-induced thymic lymphomas. |
title_short | In vitro malignant progression of cells derived from Abelson murine leukaemia virus-induced thymic lymphomas. |
title_sort | in vitro malignant progression of cells derived from abelson murine leukaemia virus-induced thymic lymphomas. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246750/ https://www.ncbi.nlm.nih.gov/pubmed/3262364 |
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