Inhibition of mitogen stimulated growth of human colon cancer cells by interferon.

Recombinant human interferon alpha inhibits growth of a human colon cancer cell line, Colo 205. To explore the mechanisms of IFN induced growth inhibition, quiescent Colo 205 cells were stimulated to proliferate in serum-free media by defined growth factors. Addition of insulin, transferrin and sele...

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Autores principales: Hamburger, A. W., Condon, M. E., O'Donnell, K.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1988
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246752/
https://www.ncbi.nlm.nih.gov/pubmed/3166905
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author Hamburger, A. W.
Condon, M. E.
O'Donnell, K.
author_facet Hamburger, A. W.
Condon, M. E.
O'Donnell, K.
author_sort Hamburger, A. W.
collection PubMed
description Recombinant human interferon alpha inhibits growth of a human colon cancer cell line, Colo 205. To explore the mechanisms of IFN induced growth inhibition, quiescent Colo 205 cells were stimulated to proliferate in serum-free media by defined growth factors. Addition of insulin, transferrin and selenium (ITS) stimulated DNA synthesis, as measured by 3H-thymidine incorporation, in a dose-dependent manner. IFN-alpha (at concentrations greater than 100 U ml-1) inhibited ITS stimulated DNA synthesis by 63%. Inhibition of cell cycle traverse was confirmed by flow cytometric analysis. Although IFN inhibited growth of ITS-treated cells, steady state levels of c-myc mRNA remained above levels observed in unstimulated cells. IFN inhibited DNA synthesis only when added prior to mitogen stimulation. IFN, added 6 h after exposure of quiescent cells to ITS, failed to inhibit cell growth. Addition of increasing concentrations of ITS failed to overcome the IFN-induced growth inhibition. These results suggest IFN may inhibit cell growth in part by antagonizing the action of growth factors. IMAGES:
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spelling pubmed-22467522009-09-10 Inhibition of mitogen stimulated growth of human colon cancer cells by interferon. Hamburger, A. W. Condon, M. E. O'Donnell, K. Br J Cancer Research Article Recombinant human interferon alpha inhibits growth of a human colon cancer cell line, Colo 205. To explore the mechanisms of IFN induced growth inhibition, quiescent Colo 205 cells were stimulated to proliferate in serum-free media by defined growth factors. Addition of insulin, transferrin and selenium (ITS) stimulated DNA synthesis, as measured by 3H-thymidine incorporation, in a dose-dependent manner. IFN-alpha (at concentrations greater than 100 U ml-1) inhibited ITS stimulated DNA synthesis by 63%. Inhibition of cell cycle traverse was confirmed by flow cytometric analysis. Although IFN inhibited growth of ITS-treated cells, steady state levels of c-myc mRNA remained above levels observed in unstimulated cells. IFN inhibited DNA synthesis only when added prior to mitogen stimulation. IFN, added 6 h after exposure of quiescent cells to ITS, failed to inhibit cell growth. Addition of increasing concentrations of ITS failed to overcome the IFN-induced growth inhibition. These results suggest IFN may inhibit cell growth in part by antagonizing the action of growth factors. IMAGES: Nature Publishing Group 1988-08 /pmc/articles/PMC2246752/ /pubmed/3166905 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Hamburger, A. W.
Condon, M. E.
O'Donnell, K.
Inhibition of mitogen stimulated growth of human colon cancer cells by interferon.
title Inhibition of mitogen stimulated growth of human colon cancer cells by interferon.
title_full Inhibition of mitogen stimulated growth of human colon cancer cells by interferon.
title_fullStr Inhibition of mitogen stimulated growth of human colon cancer cells by interferon.
title_full_unstemmed Inhibition of mitogen stimulated growth of human colon cancer cells by interferon.
title_short Inhibition of mitogen stimulated growth of human colon cancer cells by interferon.
title_sort inhibition of mitogen stimulated growth of human colon cancer cells by interferon.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246752/
https://www.ncbi.nlm.nih.gov/pubmed/3166905
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