Measurement of oestrogen receptor mRNA levels in human breast tumours.

A sensitive single-stranded hybridisation probe for the oestrogen receptor mRNA was synthesised using T3 polymerase from oestrogen receptor cDNA cloned in the Bluescript vector. This probe was used to measure oestrogen receptor mRNA in total RNA extracted from breast tumours. Oestrogen receptor mRNA...

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Autores principales: Henry, J. A., Nicholson, S., Farndon, J. R., Westley, B. R., May, F. E.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1988
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246829/
https://www.ncbi.nlm.nih.gov/pubmed/2851309
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author Henry, J. A.
Nicholson, S.
Farndon, J. R.
Westley, B. R.
May, F. E.
author_facet Henry, J. A.
Nicholson, S.
Farndon, J. R.
Westley, B. R.
May, F. E.
author_sort Henry, J. A.
collection PubMed
description A sensitive single-stranded hybridisation probe for the oestrogen receptor mRNA was synthesised using T3 polymerase from oestrogen receptor cDNA cloned in the Bluescript vector. This probe was used to measure oestrogen receptor mRNA in total RNA extracted from breast tumours. Oestrogen receptor mRNA was detected in 41 of 47 (87%) tumours whereas cytosolic oestrogen receptor protein was detected in only 18 out of 39 (46%). There was a significant correlation between the levels of the oestrogen receptor, as measured by 3H-oestradiol binding, and the oestrogen receptor mRNA. Oestrogen receptor mRNA was detected in 15 of the 21 tumours that did not contain detectable oestrogen receptor protein. This suggests that detection of the mRNA is a more sensitive means for establishing oestrogen receptor status than the radioligand oestrogen receptor assay. Oestrogen receptor mRNA was found in all histological tumour types examined. Its level was related to tumour differentiation. All the tumours that did not contain oestrogen receptor mRNA and most of the tumours that contained only low levels of oestrogen receptor mRNA were classified as grade III according to Bloom and Richardson: the median of the oestrogen receptor mRNA levels was significantly lower in this group. IMAGES:
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spelling pubmed-22468292009-09-10 Measurement of oestrogen receptor mRNA levels in human breast tumours. Henry, J. A. Nicholson, S. Farndon, J. R. Westley, B. R. May, F. E. Br J Cancer Research Article A sensitive single-stranded hybridisation probe for the oestrogen receptor mRNA was synthesised using T3 polymerase from oestrogen receptor cDNA cloned in the Bluescript vector. This probe was used to measure oestrogen receptor mRNA in total RNA extracted from breast tumours. Oestrogen receptor mRNA was detected in 41 of 47 (87%) tumours whereas cytosolic oestrogen receptor protein was detected in only 18 out of 39 (46%). There was a significant correlation between the levels of the oestrogen receptor, as measured by 3H-oestradiol binding, and the oestrogen receptor mRNA. Oestrogen receptor mRNA was detected in 15 of the 21 tumours that did not contain detectable oestrogen receptor protein. This suggests that detection of the mRNA is a more sensitive means for establishing oestrogen receptor status than the radioligand oestrogen receptor assay. Oestrogen receptor mRNA was found in all histological tumour types examined. Its level was related to tumour differentiation. All the tumours that did not contain oestrogen receptor mRNA and most of the tumours that contained only low levels of oestrogen receptor mRNA were classified as grade III according to Bloom and Richardson: the median of the oestrogen receptor mRNA levels was significantly lower in this group. IMAGES: Nature Publishing Group 1988-11 /pmc/articles/PMC2246829/ /pubmed/2851309 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Henry, J. A.
Nicholson, S.
Farndon, J. R.
Westley, B. R.
May, F. E.
Measurement of oestrogen receptor mRNA levels in human breast tumours.
title Measurement of oestrogen receptor mRNA levels in human breast tumours.
title_full Measurement of oestrogen receptor mRNA levels in human breast tumours.
title_fullStr Measurement of oestrogen receptor mRNA levels in human breast tumours.
title_full_unstemmed Measurement of oestrogen receptor mRNA levels in human breast tumours.
title_short Measurement of oestrogen receptor mRNA levels in human breast tumours.
title_sort measurement of oestrogen receptor mrna levels in human breast tumours.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2246829/
https://www.ncbi.nlm.nih.gov/pubmed/2851309
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