Use of a carbocyanine dye as a marker of functional vasculature in murine tumours.

An intravenously administered fluorescent carbocyanine dye, DiOC7(3), has been evaluated for use in conjunction with Hoechst 33342 as a marker of murine tumour vasculature. DiOC7(3) stains cells immediately adjacent to blood vessels and thus, like Hoechst 33342, outlines perfused tumour vasculature....

Descripción completa

Detalles Bibliográficos
Autores principales: Trotter, M. J., Chaplin, D. J., Olive, P. L.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1989
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2247238/
https://www.ncbi.nlm.nih.gov/pubmed/2472164
_version_ 1782150939306622976
author Trotter, M. J.
Chaplin, D. J.
Olive, P. L.
author_facet Trotter, M. J.
Chaplin, D. J.
Olive, P. L.
author_sort Trotter, M. J.
collection PubMed
description An intravenously administered fluorescent carbocyanine dye, DiOC7(3), has been evaluated for use in conjunction with Hoechst 33342 as a marker of murine tumour vasculature. DiOC7(3) stains cells immediately adjacent to blood vessels and thus, like Hoechst 33342, outlines perfused tumour vasculature. The different fluorescence excitation and emission properties of DiOC7(3) and Hoechst 33342 permit discrimination of the stains in the same tissue section. Mice tolerate a DiOC7(3) dose of 1 mg kg-1 i.v. with no ill effects. The dye has a distribution half-life in blood of 180s and staining of perivascular tumour cells is sufficiently stable to allow visualisation of vasculature for up to 30 min after DiOC7(3) injection. However, DiOC7(3) causes a 75% reduction in tumour blood flow as measured by laser Doppler techniques. Consequently, the compound appears to be most suitable as a second vascular marker, administered at some time after Hoechst 33342, to detect temporal and spatial fluctuations in tumour perfusion. IMAGES:
format Text
id pubmed-2247238
institution National Center for Biotechnology Information
language English
publishDate 1989
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-22472382009-09-10 Use of a carbocyanine dye as a marker of functional vasculature in murine tumours. Trotter, M. J. Chaplin, D. J. Olive, P. L. Br J Cancer Research Article An intravenously administered fluorescent carbocyanine dye, DiOC7(3), has been evaluated for use in conjunction with Hoechst 33342 as a marker of murine tumour vasculature. DiOC7(3) stains cells immediately adjacent to blood vessels and thus, like Hoechst 33342, outlines perfused tumour vasculature. The different fluorescence excitation and emission properties of DiOC7(3) and Hoechst 33342 permit discrimination of the stains in the same tissue section. Mice tolerate a DiOC7(3) dose of 1 mg kg-1 i.v. with no ill effects. The dye has a distribution half-life in blood of 180s and staining of perivascular tumour cells is sufficiently stable to allow visualisation of vasculature for up to 30 min after DiOC7(3) injection. However, DiOC7(3) causes a 75% reduction in tumour blood flow as measured by laser Doppler techniques. Consequently, the compound appears to be most suitable as a second vascular marker, administered at some time after Hoechst 33342, to detect temporal and spatial fluctuations in tumour perfusion. IMAGES: Nature Publishing Group 1989-05 /pmc/articles/PMC2247238/ /pubmed/2472164 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Trotter, M. J.
Chaplin, D. J.
Olive, P. L.
Use of a carbocyanine dye as a marker of functional vasculature in murine tumours.
title Use of a carbocyanine dye as a marker of functional vasculature in murine tumours.
title_full Use of a carbocyanine dye as a marker of functional vasculature in murine tumours.
title_fullStr Use of a carbocyanine dye as a marker of functional vasculature in murine tumours.
title_full_unstemmed Use of a carbocyanine dye as a marker of functional vasculature in murine tumours.
title_short Use of a carbocyanine dye as a marker of functional vasculature in murine tumours.
title_sort use of a carbocyanine dye as a marker of functional vasculature in murine tumours.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2247238/
https://www.ncbi.nlm.nih.gov/pubmed/2472164
work_keys_str_mv AT trottermj useofacarbocyaninedyeasamarkeroffunctionalvasculatureinmurinetumours
AT chaplindj useofacarbocyaninedyeasamarkeroffunctionalvasculatureinmurinetumours
AT olivepl useofacarbocyaninedyeasamarkeroffunctionalvasculatureinmurinetumours

Ejemplares similares