Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.

Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobuli...

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Detalles Bibliográficos
Autores principales: Lemoine, N. R., Mayall, E. S., Jones, T., Sheer, D., McDermid, S., Kendall-Taylor, P., Wynford-Thomas, D.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1989
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2247263/
https://www.ncbi.nlm.nih.gov/pubmed/2557880
Descripción
Sumario:Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques. IMAGES:

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