Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.

Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobuli...

Descripción completa

Detalles Bibliográficos
Autores principales: Lemoine, N. R., Mayall, E. S., Jones, T., Sheer, D., McDermid, S., Kendall-Taylor, P., Wynford-Thomas, D.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1989
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2247263/
https://www.ncbi.nlm.nih.gov/pubmed/2557880
_version_ 1782150944319864832
author Lemoine, N. R.
Mayall, E. S.
Jones, T.
Sheer, D.
McDermid, S.
Kendall-Taylor, P.
Wynford-Thomas, D.
author_facet Lemoine, N. R.
Mayall, E. S.
Jones, T.
Sheer, D.
McDermid, S.
Kendall-Taylor, P.
Wynford-Thomas, D.
author_sort Lemoine, N. R.
collection PubMed
description Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques. IMAGES:
format Text
id pubmed-2247263
institution National Center for Biotechnology Information
language English
publishDate 1989
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-22472632009-09-10 Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection. Lemoine, N. R. Mayall, E. S. Jones, T. Sheer, D. McDermid, S. Kendall-Taylor, P. Wynford-Thomas, D. Br J Cancer Research Article Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques. IMAGES: Nature Publishing Group 1989-12 /pmc/articles/PMC2247263/ /pubmed/2557880 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Lemoine, N. R.
Mayall, E. S.
Jones, T.
Sheer, D.
McDermid, S.
Kendall-Taylor, P.
Wynford-Thomas, D.
Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.
title Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.
title_full Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.
title_fullStr Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.
title_full_unstemmed Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.
title_short Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.
title_sort characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 dna transfection.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2247263/
https://www.ncbi.nlm.nih.gov/pubmed/2557880
work_keys_str_mv AT lemoinenr characterisationofhumanthyroidepithelialcellsimmortalisedinvitrobysimianvirus40dnatransfection
AT mayalles characterisationofhumanthyroidepithelialcellsimmortalisedinvitrobysimianvirus40dnatransfection
AT jonest characterisationofhumanthyroidepithelialcellsimmortalisedinvitrobysimianvirus40dnatransfection
AT sheerd characterisationofhumanthyroidepithelialcellsimmortalisedinvitrobysimianvirus40dnatransfection
AT mcdermids characterisationofhumanthyroidepithelialcellsimmortalisedinvitrobysimianvirus40dnatransfection
AT kendalltaylorp characterisationofhumanthyroidepithelialcellsimmortalisedinvitrobysimianvirus40dnatransfection
AT wynfordthomasd characterisationofhumanthyroidepithelialcellsimmortalisedinvitrobysimianvirus40dnatransfection

Ejemplares similares