Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065.

WR 1065, 2-[(minopropyl) amino] ethanethiol is an effective scavenger of free radicals. When present during irradiation it reduces cellular DNA damage as analysed by alkaline elution from filters. The same technique indicates that without irradiation, WR 1065 has no effect of DNA integrity. Using nu...

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Detalles Bibliográficos
Autores principales: Vaughan, A. T., Grdina, D. J., Meechan, P. J., Milner, A. E., Gordon, D. J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1989
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2247278/
https://www.ncbi.nlm.nih.gov/pubmed/2605099
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author Vaughan, A. T.
Grdina, D. J.
Meechan, P. J.
Milner, A. E.
Gordon, D. J.
author_facet Vaughan, A. T.
Grdina, D. J.
Meechan, P. J.
Milner, A. E.
Gordon, D. J.
author_sort Vaughan, A. T.
collection PubMed
description WR 1065, 2-[(minopropyl) amino] ethanethiol is an effective scavenger of free radicals. When present during irradiation it reduces cellular DNA damage as analysed by alkaline elution from filters. The same technique indicates that without irradiation, WR 1065 has no effect of DNA integrity. Using nucleoid analysis, where DNA damage is detected at the level of replicon clusters, WR 1065 distorts replicon supercoiling without breaking the DNA molecule. This confirmational change in nucleoid structure occurs with no detectable change in nucleoid protein content. It is proposed that perturbation of replicon supercoiling affects the process of normal DNA synthesis and strand break rejoining, allowing a longer time for the accurate repair of DNA damage. IMAGES:
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spelling pubmed-22472782009-09-10 Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065. Vaughan, A. T. Grdina, D. J. Meechan, P. J. Milner, A. E. Gordon, D. J. Br J Cancer Research Article WR 1065, 2-[(minopropyl) amino] ethanethiol is an effective scavenger of free radicals. When present during irradiation it reduces cellular DNA damage as analysed by alkaline elution from filters. The same technique indicates that without irradiation, WR 1065 has no effect of DNA integrity. Using nucleoid analysis, where DNA damage is detected at the level of replicon clusters, WR 1065 distorts replicon supercoiling without breaking the DNA molecule. This confirmational change in nucleoid structure occurs with no detectable change in nucleoid protein content. It is proposed that perturbation of replicon supercoiling affects the process of normal DNA synthesis and strand break rejoining, allowing a longer time for the accurate repair of DNA damage. IMAGES: Nature Publishing Group 1989-12 /pmc/articles/PMC2247278/ /pubmed/2605099 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Vaughan, A. T.
Grdina, D. J.
Meechan, P. J.
Milner, A. E.
Gordon, D. J.
Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065.
title Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065.
title_full Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065.
title_fullStr Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065.
title_full_unstemmed Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065.
title_short Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065.
title_sort conformational changes in chromatin structure induced by the radioprotective aminothiol, wr 1065.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2247278/
https://www.ncbi.nlm.nih.gov/pubmed/2605099
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