Loss of chromosome 11p alleles in cultured cells derived from Wilms' tumours.

Cell cultures have been produced from five Wilms' tumours. All cultures had a finite lifespan and a pattern of antigen expression which indicated that the cells were derived from the differentiated components of the tumours. No cells showed any of the expected characteristics of the putative Wi...

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Autores principales: Brown, K. W., Shaw, A. P., Poirier, V., Tyler, S. J., Berry, P. J., Mott, M. G., Maitland, N. J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1989
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2247355/
https://www.ncbi.nlm.nih.gov/pubmed/2553087
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author Brown, K. W.
Shaw, A. P.
Poirier, V.
Tyler, S. J.
Berry, P. J.
Mott, M. G.
Maitland, N. J.
author_facet Brown, K. W.
Shaw, A. P.
Poirier, V.
Tyler, S. J.
Berry, P. J.
Mott, M. G.
Maitland, N. J.
author_sort Brown, K. W.
collection PubMed
description Cell cultures have been produced from five Wilms' tumours. All cultures had a finite lifespan and a pattern of antigen expression which indicated that the cells were derived from the differentiated components of the tumours. No cells showed any of the expected characteristics of the putative Wilms' tumour stem cell. Nevertheless, in both cases where the original tumours showed a loss of heterozygosity at chromosome 11p alleles, the cultured cells also demonstrated a loss of heterozygosity. Thus these cell cultures definitely originated from Wilms' tumour tissue. The results demonstrate that cell cultures can be produced from the differentiated tissues present in Wilms' tumours and that these non-immortal cells show no 'transformed' phenotype, even though they possess the genetic changes present in the original tumour. IMAGES:
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spelling pubmed-22473552009-09-10 Loss of chromosome 11p alleles in cultured cells derived from Wilms' tumours. Brown, K. W. Shaw, A. P. Poirier, V. Tyler, S. J. Berry, P. J. Mott, M. G. Maitland, N. J. Br J Cancer Research Article Cell cultures have been produced from five Wilms' tumours. All cultures had a finite lifespan and a pattern of antigen expression which indicated that the cells were derived from the differentiated components of the tumours. No cells showed any of the expected characteristics of the putative Wilms' tumour stem cell. Nevertheless, in both cases where the original tumours showed a loss of heterozygosity at chromosome 11p alleles, the cultured cells also demonstrated a loss of heterozygosity. Thus these cell cultures definitely originated from Wilms' tumour tissue. The results demonstrate that cell cultures can be produced from the differentiated tissues present in Wilms' tumours and that these non-immortal cells show no 'transformed' phenotype, even though they possess the genetic changes present in the original tumour. IMAGES: Nature Publishing Group 1989-07 /pmc/articles/PMC2247355/ /pubmed/2553087 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Brown, K. W.
Shaw, A. P.
Poirier, V.
Tyler, S. J.
Berry, P. J.
Mott, M. G.
Maitland, N. J.
Loss of chromosome 11p alleles in cultured cells derived from Wilms' tumours.
title Loss of chromosome 11p alleles in cultured cells derived from Wilms' tumours.
title_full Loss of chromosome 11p alleles in cultured cells derived from Wilms' tumours.
title_fullStr Loss of chromosome 11p alleles in cultured cells derived from Wilms' tumours.
title_full_unstemmed Loss of chromosome 11p alleles in cultured cells derived from Wilms' tumours.
title_short Loss of chromosome 11p alleles in cultured cells derived from Wilms' tumours.
title_sort loss of chromosome 11p alleles in cultured cells derived from wilms' tumours.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2247355/
https://www.ncbi.nlm.nih.gov/pubmed/2553087
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