Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris

Exendin-4, a peptide analogue of glucagon-like peptide-1 (GLP-1), has been developed for treatment of type 2 diabetes. Herein, the secretive exendin-4 fusion protein, expressed by methanol induction in Pichia pastoris system, was purified to homogeneity by chromatography followed by enterokinase cle...

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Detalles Bibliográficos
Autores principales: Zhou, Jin, Chu, Ju, Wang, Yong-Hong, Wang, Hui, Zhuang, Ying-Ping, Zhang, Si-Liang
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2007
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248218/
https://www.ncbi.nlm.nih.gov/pubmed/18060365
http://dx.doi.org/10.1007/s10529-007-9610-4
Descripción
Sumario:Exendin-4, a peptide analogue of glucagon-like peptide-1 (GLP-1), has been developed for treatment of type 2 diabetes. Herein, the secretive exendin-4 fusion protein, expressed by methanol induction in Pichia pastoris system, was purified to homogeneity by chromatography followed by enterokinase cleavage of the fusion protein and subsequent purification of the recombinant exendin-4. Purity of the recombinant exendin-4 was 95.6%. Bioactivity assay revealed that it had glucose-lowering and insulin-releasing action in vivo.

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