Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris

Exendin-4, a peptide analogue of glucagon-like peptide-1 (GLP-1), has been developed for treatment of type 2 diabetes. Herein, the secretive exendin-4 fusion protein, expressed by methanol induction in Pichia pastoris system, was purified to homogeneity by chromatography followed by enterokinase cle...

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Detalles Bibliográficos
Autores principales: Zhou, Jin, Chu, Ju, Wang, Yong-Hong, Wang, Hui, Zhuang, Ying-Ping, Zhang, Si-Liang
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2007
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248218/
https://www.ncbi.nlm.nih.gov/pubmed/18060365
http://dx.doi.org/10.1007/s10529-007-9610-4
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author Zhou, Jin
Chu, Ju
Wang, Yong-Hong
Wang, Hui
Zhuang, Ying-Ping
Zhang, Si-Liang
author_facet Zhou, Jin
Chu, Ju
Wang, Yong-Hong
Wang, Hui
Zhuang, Ying-Ping
Zhang, Si-Liang
author_sort Zhou, Jin
collection PubMed
description Exendin-4, a peptide analogue of glucagon-like peptide-1 (GLP-1), has been developed for treatment of type 2 diabetes. Herein, the secretive exendin-4 fusion protein, expressed by methanol induction in Pichia pastoris system, was purified to homogeneity by chromatography followed by enterokinase cleavage of the fusion protein and subsequent purification of the recombinant exendin-4. Purity of the recombinant exendin-4 was 95.6%. Bioactivity assay revealed that it had glucose-lowering and insulin-releasing action in vivo.
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spelling pubmed-22482182008-02-20 Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris Zhou, Jin Chu, Ju Wang, Yong-Hong Wang, Hui Zhuang, Ying-Ping Zhang, Si-Liang Biotechnol Lett Original Research Paper Exendin-4, a peptide analogue of glucagon-like peptide-1 (GLP-1), has been developed for treatment of type 2 diabetes. Herein, the secretive exendin-4 fusion protein, expressed by methanol induction in Pichia pastoris system, was purified to homogeneity by chromatography followed by enterokinase cleavage of the fusion protein and subsequent purification of the recombinant exendin-4. Purity of the recombinant exendin-4 was 95.6%. Bioactivity assay revealed that it had glucose-lowering and insulin-releasing action in vivo. Springer Netherlands 2007-12-01 2008-04 /pmc/articles/PMC2248218/ /pubmed/18060365 http://dx.doi.org/10.1007/s10529-007-9610-4 Text en © The Author(s) 2007
spellingShingle Original Research Paper
Zhou, Jin
Chu, Ju
Wang, Yong-Hong
Wang, Hui
Zhuang, Ying-Ping
Zhang, Si-Liang
Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris
title Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris
title_full Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris
title_fullStr Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris
title_full_unstemmed Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris
title_short Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris
title_sort purification and bioactivity of exendin-4, a peptide analogue of glp-1, expressed in pichia pastoris
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248218/
https://www.ncbi.nlm.nih.gov/pubmed/18060365
http://dx.doi.org/10.1007/s10529-007-9610-4
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