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Recombineering with tolC as a Selectable/Counter-selectable Marker: remodeling the rRNA Operons of Escherichia coli
This work describes the novel use of tolC as a selectable/counter-selectable marker for the facile modification of DNA in Escherichia coli. Expression of TolC (an outer membrane protein) confers relative resistance to toxic small molecules, while its absence renders the cell tolerant to colicin E1....
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| Formato: | Texto |
| Lenguaje: | English |
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Oxford University Press
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248734/ https://www.ncbi.nlm.nih.gov/pubmed/18084036 http://dx.doi.org/10.1093/nar/gkm1084 |