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Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves

tie-dyed1 (tdy1) and sucrose export defective1 (sxd1) are recessive maize (Zea mays) mutants with nonclonal chlorotic leaf sectors that hyperaccumulate starch and soluble sugars. In addition, both mutants display similar growth-related defects such as reduced plant height and inflorescence developme...

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Autores principales: Ma, Yi, Baker, R. Frank, Magallanes-Lundback, Maria, DellaPenna, Dean, Braun, David M.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2249615/
https://www.ncbi.nlm.nih.gov/pubmed/17924136
http://dx.doi.org/10.1007/s00425-007-0636-6
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author Ma, Yi
Baker, R. Frank
Magallanes-Lundback, Maria
DellaPenna, Dean
Braun, David M.
author_facet Ma, Yi
Baker, R. Frank
Magallanes-Lundback, Maria
DellaPenna, Dean
Braun, David M.
author_sort Ma, Yi
collection PubMed
description tie-dyed1 (tdy1) and sucrose export defective1 (sxd1) are recessive maize (Zea mays) mutants with nonclonal chlorotic leaf sectors that hyperaccumulate starch and soluble sugars. In addition, both mutants display similar growth-related defects such as reduced plant height and inflorescence development due to the retention of carbohydrates in leaves. As tdy1 and sxd1 are the only variegated leaf mutants known to accumulate carbohydrates in any plant, we investigated whether Tdy1 and Sxd1 function in the same pathway. Using aniline blue staining for callose and transmission electron microscopy to inspect plasmodesmatal ultrastructure, we determined that tdy1 does not have any physical blockage or alteration along the symplastic transport pathway as found in sxd1 mutants. To test whether the two genes function in the same genetic pathway, we constructed F(2) families segregating both mutations. Double mutant plants showed an additive interaction for growth related phenotypes and soluble sugar accumulation, and expressed the leaf variegation pattern of both single mutants indicating that Tdy1 and Sxd1 act in separate genetic pathways. Although sxd1 mutants lack tocopherols, we determined that tdy1 mutants have wild-type tocopherol levels, indicating that Tdy1 does not function in the same biochemical pathway as Sxd1. From these and other data we conclude that Tdy1 and Sxd1 function independently to promote carbon export from leaves. Our genetic and cytological studies implicate Tdy1 functioning in veins, and a model discussing possible functions of TDY1 is presented. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00425-007-0636-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-22496152008-02-22 Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves Ma, Yi Baker, R. Frank Magallanes-Lundback, Maria DellaPenna, Dean Braun, David M. Planta Original Article tie-dyed1 (tdy1) and sucrose export defective1 (sxd1) are recessive maize (Zea mays) mutants with nonclonal chlorotic leaf sectors that hyperaccumulate starch and soluble sugars. In addition, both mutants display similar growth-related defects such as reduced plant height and inflorescence development due to the retention of carbohydrates in leaves. As tdy1 and sxd1 are the only variegated leaf mutants known to accumulate carbohydrates in any plant, we investigated whether Tdy1 and Sxd1 function in the same pathway. Using aniline blue staining for callose and transmission electron microscopy to inspect plasmodesmatal ultrastructure, we determined that tdy1 does not have any physical blockage or alteration along the symplastic transport pathway as found in sxd1 mutants. To test whether the two genes function in the same genetic pathway, we constructed F(2) families segregating both mutations. Double mutant plants showed an additive interaction for growth related phenotypes and soluble sugar accumulation, and expressed the leaf variegation pattern of both single mutants indicating that Tdy1 and Sxd1 act in separate genetic pathways. Although sxd1 mutants lack tocopherols, we determined that tdy1 mutants have wild-type tocopherol levels, indicating that Tdy1 does not function in the same biochemical pathway as Sxd1. From these and other data we conclude that Tdy1 and Sxd1 function independently to promote carbon export from leaves. Our genetic and cytological studies implicate Tdy1 functioning in veins, and a model discussing possible functions of TDY1 is presented. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00425-007-0636-6) contains supplementary material, which is available to authorized users. Springer-Verlag 2007-10-09 2008-02 /pmc/articles/PMC2249615/ /pubmed/17924136 http://dx.doi.org/10.1007/s00425-007-0636-6 Text en © Springer-Verlag 2007
spellingShingle Original Article
Ma, Yi
Baker, R. Frank
Magallanes-Lundback, Maria
DellaPenna, Dean
Braun, David M.
Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves
title Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves
title_full Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves
title_fullStr Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves
title_full_unstemmed Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves
title_short Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves
title_sort tie-dyed1 and sucrose export defective1 act independently to promote carbohydrate export from maize leaves
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2249615/
https://www.ncbi.nlm.nih.gov/pubmed/17924136
http://dx.doi.org/10.1007/s00425-007-0636-6
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