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Quantitative comparison of DNA detection by GFP-lac repressor tagging, fluorescence in situ hybridization and immunostaining

BACKGROUND: GFP-fusion proteins and immunostaining are methods broadly applied to investigate the three-dimensional organization of cells and cell nuclei, the latter often studied in addition by fluorescence in situ hybridization (FISH). Direct comparisons of these detection methods are scarce, howe...

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Detalles Bibliográficos
Autores principales: Kim, Il-Han, Nagel, Jens, Otten, Simone, Knerr, Boris, Eils, Roland, Rohr, Karl, Dietzel, Steffen
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2254608/
https://www.ncbi.nlm.nih.gov/pubmed/18096031
http://dx.doi.org/10.1186/1472-6750-7-92