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Cyclic GMP in the pig vitreous and retina after experimental retinal detachment
PURPOSE: Earlier studies have revealed a decreased level of cGMP in vitreous fluid obtained from patients with a retinal detachment. To further investigate this phenomenon, we developed an experimental retinal detachment model in pigs. METHODS: Experimental unilateral retinal detachments were induce...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Molecular Vision
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2254957/ https://www.ncbi.nlm.nih.gov/pubmed/18334939 |
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author | Diederen, Roselie M.H. La Heij, Ellen C. Lemmens, Marijke A.M. Kijlstra, Aize de Vente, Jan Hendrikse, Fred |
author_facet | Diederen, Roselie M.H. La Heij, Ellen C. Lemmens, Marijke A.M. Kijlstra, Aize de Vente, Jan Hendrikse, Fred |
author_sort | Diederen, Roselie M.H. |
collection | PubMed |
description | PURPOSE: Earlier studies have revealed a decreased level of cGMP in vitreous fluid obtained from patients with a retinal detachment. To further investigate this phenomenon, we developed an experimental retinal detachment model in pigs. METHODS: Experimental unilateral retinal detachments were induced in pig eyes by subretinal injection of 0.25% sodium hyaluronate. Fourteen days later the vitreous and retinas were analyzed for cGMP expression. Following enucleation, the retinas were incubated in the presence of a nonselective phosphodiesterase inhibitor (IBMX), and the particulate guanylyl cyclase stimulator atrial natriuretic peptide (ANP) or the soluble guanylyl cyclase stimulator sodium nitroprusside (SNP). cGMP was visualized in retinal wholemounts by immunochemistry combined with a computer based stereology system. cGMP levels in vitreous were determined by ELISA. RESULTS: The mean vitreous cGMP level in pig eyes with a retinal detachment (1.45 pmol/ml) was significantly lower compared to the mean level of cGMP in healthy pig eyes (4.61 pmol/ml; p=0.028 was considered significant). In the inner retina, ANP as well as SNP induced cGMP immunoreactivity in both detached and healthy retinas. After incubation with ANP, cGMP could also be detected in the outer nuclear layer of the detached retina, whereas this was not the case in the normal retina. CONCLUSIONS: Experimental retinal detachment in the pig eye leads to a decrease of cGMP levels in vitreous similar to that observed in clinical studies. This model may be helpful to analyze the mechanisms involved in cGMP dynamics following retinal detachment. |
format | Text |
id | pubmed-2254957 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-22549572008-03-11 Cyclic GMP in the pig vitreous and retina after experimental retinal detachment Diederen, Roselie M.H. La Heij, Ellen C. Lemmens, Marijke A.M. Kijlstra, Aize de Vente, Jan Hendrikse, Fred Mol Vis Research Article PURPOSE: Earlier studies have revealed a decreased level of cGMP in vitreous fluid obtained from patients with a retinal detachment. To further investigate this phenomenon, we developed an experimental retinal detachment model in pigs. METHODS: Experimental unilateral retinal detachments were induced in pig eyes by subretinal injection of 0.25% sodium hyaluronate. Fourteen days later the vitreous and retinas were analyzed for cGMP expression. Following enucleation, the retinas were incubated in the presence of a nonselective phosphodiesterase inhibitor (IBMX), and the particulate guanylyl cyclase stimulator atrial natriuretic peptide (ANP) or the soluble guanylyl cyclase stimulator sodium nitroprusside (SNP). cGMP was visualized in retinal wholemounts by immunochemistry combined with a computer based stereology system. cGMP levels in vitreous were determined by ELISA. RESULTS: The mean vitreous cGMP level in pig eyes with a retinal detachment (1.45 pmol/ml) was significantly lower compared to the mean level of cGMP in healthy pig eyes (4.61 pmol/ml; p=0.028 was considered significant). In the inner retina, ANP as well as SNP induced cGMP immunoreactivity in both detached and healthy retinas. After incubation with ANP, cGMP could also be detected in the outer nuclear layer of the detached retina, whereas this was not the case in the normal retina. CONCLUSIONS: Experimental retinal detachment in the pig eye leads to a decrease of cGMP levels in vitreous similar to that observed in clinical studies. This model may be helpful to analyze the mechanisms involved in cGMP dynamics following retinal detachment. Molecular Vision 2008-02-04 /pmc/articles/PMC2254957/ /pubmed/18334939 Text en Copyright © 2008 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Diederen, Roselie M.H. La Heij, Ellen C. Lemmens, Marijke A.M. Kijlstra, Aize de Vente, Jan Hendrikse, Fred Cyclic GMP in the pig vitreous and retina after experimental retinal detachment |
title | Cyclic GMP in the pig vitreous and retina after experimental retinal detachment |
title_full | Cyclic GMP in the pig vitreous and retina after experimental retinal detachment |
title_fullStr | Cyclic GMP in the pig vitreous and retina after experimental retinal detachment |
title_full_unstemmed | Cyclic GMP in the pig vitreous and retina after experimental retinal detachment |
title_short | Cyclic GMP in the pig vitreous and retina after experimental retinal detachment |
title_sort | cyclic gmp in the pig vitreous and retina after experimental retinal detachment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2254957/ https://www.ncbi.nlm.nih.gov/pubmed/18334939 |
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