Protocol: Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway(® )TOPO vector system

BACKGROUND: In plant functional genomic studies, gene cloning into binary vectors for plant transformation is a routine procedure. Traditionally, gene cloning has relied on restriction enzyme digestion and ligation. In recent years, however, Gateway(® )cloning technology (Invitrogen Co.) has develop...

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Detalles Bibliográficos
Autores principales: Xu, Ruqiang, Li, Qingshun Quinn
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2257932/
https://www.ncbi.nlm.nih.gov/pubmed/18211693
http://dx.doi.org/10.1186/1746-4811-4-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2257932/
https://www.ncbi.nlm.nih.gov/pubmed/18211693
http://dx.doi.org/10.1186/1746-4811-4-4

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