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Protocol: Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway(® )TOPO vector system
BACKGROUND: In plant functional genomic studies, gene cloning into binary vectors for plant transformation is a routine procedure. Traditionally, gene cloning has relied on restriction enzyme digestion and ligation. In recent years, however, Gateway(® )cloning technology (Invitrogen Co.) has develop...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
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BioMed Central
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2257932/ https://www.ncbi.nlm.nih.gov/pubmed/18211693 http://dx.doi.org/10.1186/1746-4811-4-4 |