Analysis of molecular inversion probe performance for allele copy number determination

We have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 μg) by more than 25-fold (to 75 ng total genomic DNA), higher overall...

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Detalles Bibliográficos
Autores principales: Wang, Yuker, Moorhead, Martin, Karlin-Neumann, George, Wang, Nicholas J, Ireland, James, Lin, Steven, Chen, Chunnuan, Heiser, Laura M, Chin, Koei, Esserman, Laura, Gray, Joe W, Spellman, Paul T, Faham, Malek
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2258201/
https://www.ncbi.nlm.nih.gov/pubmed/18028543
http://dx.doi.org/10.1186/gb-2007-8-11-r246
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author Wang, Yuker
Moorhead, Martin
Karlin-Neumann, George
Wang, Nicholas J
Ireland, James
Lin, Steven
Chen, Chunnuan
Heiser, Laura M
Chin, Koei
Esserman, Laura
Gray, Joe W
Spellman, Paul T
Faham, Malek
author_facet Wang, Yuker
Moorhead, Martin
Karlin-Neumann, George
Wang, Nicholas J
Ireland, James
Lin, Steven
Chen, Chunnuan
Heiser, Laura M
Chin, Koei
Esserman, Laura
Gray, Joe W
Spellman, Paul T
Faham, Malek
author_sort Wang, Yuker
collection PubMed
description We have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 μg) by more than 25-fold (to 75 ng total genomic DNA), higher overall precision resulting in one order of magnitude lower false positive rate, and greater dynamic range with accurate absolute copy number up to 60 copies.
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spelling pubmed-22582012008-02-28 Analysis of molecular inversion probe performance for allele copy number determination Wang, Yuker Moorhead, Martin Karlin-Neumann, George Wang, Nicholas J Ireland, James Lin, Steven Chen, Chunnuan Heiser, Laura M Chin, Koei Esserman, Laura Gray, Joe W Spellman, Paul T Faham, Malek Genome Biol Method We have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 μg) by more than 25-fold (to 75 ng total genomic DNA), higher overall precision resulting in one order of magnitude lower false positive rate, and greater dynamic range with accurate absolute copy number up to 60 copies. BioMed Central 2007 2007-11-20 /pmc/articles/PMC2258201/ /pubmed/18028543 http://dx.doi.org/10.1186/gb-2007-8-11-r246 Text en Copyright © 2007 Wang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Wang, Yuker
Moorhead, Martin
Karlin-Neumann, George
Wang, Nicholas J
Ireland, James
Lin, Steven
Chen, Chunnuan
Heiser, Laura M
Chin, Koei
Esserman, Laura
Gray, Joe W
Spellman, Paul T
Faham, Malek
Analysis of molecular inversion probe performance for allele copy number determination
title Analysis of molecular inversion probe performance for allele copy number determination
title_full Analysis of molecular inversion probe performance for allele copy number determination
title_fullStr Analysis of molecular inversion probe performance for allele copy number determination
title_full_unstemmed Analysis of molecular inversion probe performance for allele copy number determination
title_short Analysis of molecular inversion probe performance for allele copy number determination
title_sort analysis of molecular inversion probe performance for allele copy number determination
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2258201/
https://www.ncbi.nlm.nih.gov/pubmed/18028543
http://dx.doi.org/10.1186/gb-2007-8-11-r246
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