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Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate

BACKGROUND: Phosphoinositides play a central role in regulating processes at intracellular membranes. In yeast, a large number of phospholipid biosynthetic enzymes use a common mechanism for transcriptional regulation. Yet, how the expression of genes encoding lipid kinases and phosphatases is regul...

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Detalles Bibliográficos
Autores principales: Knödler, Andreas, Konrad, Gerlinde, Mayinger, Peter
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2258305/
https://www.ncbi.nlm.nih.gov/pubmed/18226253
http://dx.doi.org/10.1186/1471-2199-9-16
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author Knödler, Andreas
Konrad, Gerlinde
Mayinger, Peter
author_facet Knödler, Andreas
Konrad, Gerlinde
Mayinger, Peter
author_sort Knödler, Andreas
collection PubMed
description BACKGROUND: Phosphoinositides play a central role in regulating processes at intracellular membranes. In yeast, a large number of phospholipid biosynthetic enzymes use a common mechanism for transcriptional regulation. Yet, how the expression of genes encoding lipid kinases and phosphatases is regulated remains unknown. RESULTS: Here we show that the expression of lipid phosphatase Sac1p in the yeast Saccharomyces cerevisiae is regulated in response to changes in phosphatidylinositol-4-phosphate (PI(4)P) concentrations. Unlike genes encoding enzymes involved in phospholipid biosynthesis, expression of the SAC1 gene is independent of inositol levels. We identified a novel 9-bp motif within the 5' untranslated region (5'-UTR) of SAC1 that is responsible for PI(4)P-mediated regulation. Upregulation of SAC1 promoter activity correlates with elevated levels of Sac1 protein levels. CONCLUSION: Regulation of Sac1p expression via the concentration of its major substrate PI(4)P ensures proper maintenance of compartment-specific pools of PI(4)P.
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spelling pubmed-22583052008-02-29 Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate Knödler, Andreas Konrad, Gerlinde Mayinger, Peter BMC Mol Biol Research Article BACKGROUND: Phosphoinositides play a central role in regulating processes at intracellular membranes. In yeast, a large number of phospholipid biosynthetic enzymes use a common mechanism for transcriptional regulation. Yet, how the expression of genes encoding lipid kinases and phosphatases is regulated remains unknown. RESULTS: Here we show that the expression of lipid phosphatase Sac1p in the yeast Saccharomyces cerevisiae is regulated in response to changes in phosphatidylinositol-4-phosphate (PI(4)P) concentrations. Unlike genes encoding enzymes involved in phospholipid biosynthesis, expression of the SAC1 gene is independent of inositol levels. We identified a novel 9-bp motif within the 5' untranslated region (5'-UTR) of SAC1 that is responsible for PI(4)P-mediated regulation. Upregulation of SAC1 promoter activity correlates with elevated levels of Sac1 protein levels. CONCLUSION: Regulation of Sac1p expression via the concentration of its major substrate PI(4)P ensures proper maintenance of compartment-specific pools of PI(4)P. BioMed Central 2008-01-28 /pmc/articles/PMC2258305/ /pubmed/18226253 http://dx.doi.org/10.1186/1471-2199-9-16 Text en Copyright © 2008 Knödler et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Knödler, Andreas
Konrad, Gerlinde
Mayinger, Peter
Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
title Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
title_full Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
title_fullStr Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
title_full_unstemmed Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
title_short Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
title_sort expression of yeast lipid phosphatase sac1p is regulated by phosphatidylinositol-4-phosphate
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2258305/
https://www.ncbi.nlm.nih.gov/pubmed/18226253
http://dx.doi.org/10.1186/1471-2199-9-16
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