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Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
BACKGROUND: Phosphoinositides play a central role in regulating processes at intracellular membranes. In yeast, a large number of phospholipid biosynthetic enzymes use a common mechanism for transcriptional regulation. Yet, how the expression of genes encoding lipid kinases and phosphatases is regul...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2258305/ https://www.ncbi.nlm.nih.gov/pubmed/18226253 http://dx.doi.org/10.1186/1471-2199-9-16 |
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author | Knödler, Andreas Konrad, Gerlinde Mayinger, Peter |
author_facet | Knödler, Andreas Konrad, Gerlinde Mayinger, Peter |
author_sort | Knödler, Andreas |
collection | PubMed |
description | BACKGROUND: Phosphoinositides play a central role in regulating processes at intracellular membranes. In yeast, a large number of phospholipid biosynthetic enzymes use a common mechanism for transcriptional regulation. Yet, how the expression of genes encoding lipid kinases and phosphatases is regulated remains unknown. RESULTS: Here we show that the expression of lipid phosphatase Sac1p in the yeast Saccharomyces cerevisiae is regulated in response to changes in phosphatidylinositol-4-phosphate (PI(4)P) concentrations. Unlike genes encoding enzymes involved in phospholipid biosynthesis, expression of the SAC1 gene is independent of inositol levels. We identified a novel 9-bp motif within the 5' untranslated region (5'-UTR) of SAC1 that is responsible for PI(4)P-mediated regulation. Upregulation of SAC1 promoter activity correlates with elevated levels of Sac1 protein levels. CONCLUSION: Regulation of Sac1p expression via the concentration of its major substrate PI(4)P ensures proper maintenance of compartment-specific pools of PI(4)P. |
format | Text |
id | pubmed-2258305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22583052008-02-29 Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate Knödler, Andreas Konrad, Gerlinde Mayinger, Peter BMC Mol Biol Research Article BACKGROUND: Phosphoinositides play a central role in regulating processes at intracellular membranes. In yeast, a large number of phospholipid biosynthetic enzymes use a common mechanism for transcriptional regulation. Yet, how the expression of genes encoding lipid kinases and phosphatases is regulated remains unknown. RESULTS: Here we show that the expression of lipid phosphatase Sac1p in the yeast Saccharomyces cerevisiae is regulated in response to changes in phosphatidylinositol-4-phosphate (PI(4)P) concentrations. Unlike genes encoding enzymes involved in phospholipid biosynthesis, expression of the SAC1 gene is independent of inositol levels. We identified a novel 9-bp motif within the 5' untranslated region (5'-UTR) of SAC1 that is responsible for PI(4)P-mediated regulation. Upregulation of SAC1 promoter activity correlates with elevated levels of Sac1 protein levels. CONCLUSION: Regulation of Sac1p expression via the concentration of its major substrate PI(4)P ensures proper maintenance of compartment-specific pools of PI(4)P. BioMed Central 2008-01-28 /pmc/articles/PMC2258305/ /pubmed/18226253 http://dx.doi.org/10.1186/1471-2199-9-16 Text en Copyright © 2008 Knödler et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Knödler, Andreas Konrad, Gerlinde Mayinger, Peter Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate |
title | Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate |
title_full | Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate |
title_fullStr | Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate |
title_full_unstemmed | Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate |
title_short | Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate |
title_sort | expression of yeast lipid phosphatase sac1p is regulated by phosphatidylinositol-4-phosphate |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2258305/ https://www.ncbi.nlm.nih.gov/pubmed/18226253 http://dx.doi.org/10.1186/1471-2199-9-16 |
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