Cargando…

A real-time PCR assay for the detection and quantitation of Campylobacter jejuni using SYBR Green I and the LightCycler.

Campylobacter jejuni is recognized as a leading human food-borne pathogen. Traditional biochemical identification for C. jejuni is not reliable due to special growth requirements and the possibility that this bacterium can enter a viable but nonculturable (VNC) state. Nucleic acid-based tests have e...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yang, Chengbo, Jiang, Yuan, Huang, Kehe, Zhu, Changqing, Yin, Yulong, Gong, Jian-Hua, Yu, Hai
Formato:	Texto
Lenguaje:	English
Publicado:	Yale Journal of Biology and Medicine 2004
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2259121/ https://www.ncbi.nlm.nih.gov/pubmed/15989741

Ejemplares similares

LightCycler SeptiFast in early diagnosis of sepsis: our experience
por: Raineri, SM, et al.
Publicado: (2009)

Variability and cost implications of three generations of the Roche LightCycler® 480
por: Dullaert-de Boer, Maria, et al.
Publicado: (2018)

A novel FRET pair for detection of parallel DNA triplexes by the LightCycler
por: Schneider, Uffe V, et al.
Publicado: (2010)

Correction: Variability and cost implications of three generations of the Roche LightCycler® 480
por: Dullaert-de Boer, Maria, et al.
Publicado: (2018)

Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture
por: Burrows, Julie, et al.
Publicado: (2002)

LightCycler SeptiFast assay as a tool for the rapid diagnosis of sepsis in patients receiving antimicrobial therapy
por: Vince, Adriana, et al.
Publicado: (2008)

Analytical Performance of the Roche LightCycler® Mycobacterium Detection Kit for the Diagnosis of Clinically Important Mycobacterial Species
por: Omar, Shaheed V., et al.
Publicado: (2011)

Monkeypox virus detection in rodents using real-time 3′-minor groove binder TaqMan® assays on the Roche LightCycler
por: Kulesh, David A, et al.
Publicado: (2004)

Evaluation of Type-Specific Real-Time PCR Assays Using the LightCycler and J.B.A.I.D.S. for Detection of Adenoviruses in Species HAdV-C
por: Jones, Morris S., et al.
Publicado: (2011)

LightCycler SeptiFast technology in patients with solid malignancies: clinical utility for rapid etiologic diagnosis of sepsis
por: Dubská, Lenka, et al.
Publicado: (2012)

Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler(® )Real-time PCR
por: Kabamba-Mukadi, Benoît, et al.
Publicado: (2005)

Accuracy of LightCycler® SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review protocol
por: Dark, Paul, et al.
Publicado: (2012)

Methicillin-resistant Staphylococcus aureus in Nasal Surveillance Swabs at an Intensive Care Unit: An Evaluation of the LightCycler MRSA Advanced Test
por: Huh, Hee Jin, et al.
Publicado: (2012)

Rapid Genotyping of the Human Renin (REN) Gene by the LightCycler® Instrument: Identification of Unexpected Nucleotide Substitutions within the Selected Hybridization Probe Area
por: Wee, Line, et al.
Publicado: (2010)

Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses
por: Sultani, Mozhdeh, et al.
Publicado: (2015)

Rapid identification of Bordetella pertussis pertactin gene variants using LightCycler real-time polymerase chain reaction combined with melting curve analysis and gel electrophoresis.
por: Mäkinen, J, et al.
Publicado: (2001)

Development of a SYBR Green based real-time PCR assay for detection and quantitation of canine parvovirus in faecal samples
por: Kumar, Manoj, et al.
Publicado: (2010)

Sensitive SYBR Green—Real Time PCR for the Detection and Quantitation of Avian Rotavirus A
por: De la Torre, David, et al.
Publicado: (2018)

Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
por: García, Alfredo, et al.
Publicado: (2013)

Development of a Pan-Filoviridae SYBR Green qPCR Assay for Biosurveillance Studies in Bats
por: Coertse, Jessica, et al.
Publicado: (2023)

Quantification of Hantaan Virus with a SYBR Green Ⅰ-Based One-Step qRT-PCR Assay
por: Jiang, Wei, et al.
Publicado: (2013)

The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirus
por: Wan, Chunhe, et al.
Publicado: (2011)

Detection of Campylobacter jejuni in Lizard Faeces from Central Australia Using Quantitative PCR
por: Whiley, Harriet, et al.
Publicado: (2016)

SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
por: Eischeid, Anne C
Publicado: (2011)

Correction: Quantification of Hantaan Virus with a SYBR Green Ⅰ-Based One-Step qRT-PCR Assay
por: Jiang, Wei, et al.
Publicado: (2013)

Development of an SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus
por: Wang, Yong, et al.
Publicado: (2020)

Detection of herpes simplex virus 2: a SYBR-Green-based real-time PCR assay
por: Shaha, Modhusudon, et al.
Publicado: (2021)

TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR
por: Zhang, Qian, et al.
Publicado: (2015)

MULTIPLEX SYBR(®) GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS
por: Staggemeier, Rodrigo, et al.
Publicado: (2014)

Development of a One-Step SYBR Green I Real-Time RT-PCR Assay for the Detection and Quantitation of Araraquara and Rio Mamore Hantavirus
por: Machado, Alex Martins, et al.
Publicado: (2013)

A SYBR Green I based real time RT-PCR assay for specific detection and quantitation of Peste des petits ruminants virus
por: Abera, Tsegalem, et al.
Publicado: (2014)

Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses
por: Lin, Su, et al.
Publicado: (2019)

A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition
por: Scipioni, Alexandra, et al.
Publicado: (2008)

Development of a real time PCR assay using SYBR Green chemistry for bovine leukemia virus detection
por: Rama, Gonzalo, et al.
Publicado: (2011)

Diagnosis of Barmah Forest Virus Infection by a Nested Real-Time SYBR Green RT-PCR Assay
por: Hueston, Linda, et al.
Publicado: (2013)

Development of New PCR Assay with SYBR Green I for Detection of Mycoplasma, Acholeplasma, and Ureaplasma sp. in Cell Cultures
por: Krzysztoń-Russjan, Jolanta, et al.
Publicado: (2021)

Quantitative RNA-seq analysis of the Campylobacter jejuni transcriptome
por: Chaudhuri, Roy R., et al.
Publicado: (2011)

Comparative Analysis of Cultural Isolation and Pcr Based Assay for Detection of Campylobacter Jejuni In Food and Faecal Samples
por: Singh, Harkanwaldeep, et al.
Publicado: (2011)

Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay
por: Sierra-Arguello, Yuli Melisa, et al.
Publicado: (2018)

Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses
por: Hosmillo, Myra D.T., et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_btl3mi0hpb0rcg0atrkgjn861i