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High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis
BACKGROUND: Combination of CHD (chromo-helicase-DNA binding protein)-specific polymerase chain reaction (PCR) with electrophoresis (PCR/electrophoresis) is the most common avian molecular sexing technique but it is lab-intensive and gel-required. Gender determination often fails when the difference...
Autores principales: | Chang, Hsueh-Wei, Cheng, Chun-An, Gu, De-Leung, Chang, Chia-Che, Su, San-Hua, Wen, Cheng-Hao, Chou, Yii-Cheng, Chou, Ta-Ching, Yao, Cheng-Te, Tsai, Chi-Li, Cheng, Chien-Chung |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2259332/ https://www.ncbi.nlm.nih.gov/pubmed/18269737 http://dx.doi.org/10.1186/1472-6750-8-12 |
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