Transduction of the rat brain by Bovine Herpesvirus 4

Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFPΔTK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat glioma cells. BoHV-4EGFPΔTK was injected into the lateral ventricle of the rat brain. Hist...

Descripción completa

Detalles Bibliográficos
Autores principales: Redaelli, Marco, Cavaggioni, Andrea, Mucignat-Caretta, Carla, Cavirani, Sandro, Caretta, Antonio, Donofrio, Gaetano
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Short Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2259350/
https://www.ncbi.nlm.nih.gov/pubmed/18267037
http://dx.doi.org/10.1186/1479-0556-6-6
Descripción
Sumario:Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFPΔTK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat glioma cells. BoHV-4EGFPΔTK was injected into the lateral ventricle of the rat brain. Histology and immunohistochemistry showed that ependymal and rostral migratory stream cells were transduced while neurons were not. Clinical scores, evaluated for 90 days, indicated that the virus was non neuropathogenic, suggesting this virus is a suitable vector for brain tumor gene therapy.

Ejemplares similares