Transduction of the rat brain by Bovine Herpesvirus 4

Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFPΔTK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat glioma cells. BoHV-4EGFPΔTK was injected into the lateral ventricle of the rat brain. Hist...

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Autores principales: Redaelli, Marco, Cavaggioni, Andrea, Mucignat-Caretta, Carla, Cavirani, Sandro, Caretta, Antonio, Donofrio, Gaetano
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Short Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2259350/
https://www.ncbi.nlm.nih.gov/pubmed/18267037
http://dx.doi.org/10.1186/1479-0556-6-6
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author Redaelli, Marco
Cavaggioni, Andrea
Mucignat-Caretta, Carla
Cavirani, Sandro
Caretta, Antonio
Donofrio, Gaetano
author_facet Redaelli, Marco
Cavaggioni, Andrea
Mucignat-Caretta, Carla
Cavirani, Sandro
Caretta, Antonio
Donofrio, Gaetano
author_sort Redaelli, Marco
collection PubMed
description Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFPΔTK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat glioma cells. BoHV-4EGFPΔTK was injected into the lateral ventricle of the rat brain. Histology and immunohistochemistry showed that ependymal and rostral migratory stream cells were transduced while neurons were not. Clinical scores, evaluated for 90 days, indicated that the virus was non neuropathogenic, suggesting this virus is a suitable vector for brain tumor gene therapy.
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spelling pubmed-22593502008-03-04 Transduction of the rat brain by Bovine Herpesvirus 4 Redaelli, Marco Cavaggioni, Andrea Mucignat-Caretta, Carla Cavirani, Sandro Caretta, Antonio Donofrio, Gaetano Genet Vaccines Ther Short Paper Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFPΔTK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat glioma cells. BoHV-4EGFPΔTK was injected into the lateral ventricle of the rat brain. Histology and immunohistochemistry showed that ependymal and rostral migratory stream cells were transduced while neurons were not. Clinical scores, evaluated for 90 days, indicated that the virus was non neuropathogenic, suggesting this virus is a suitable vector for brain tumor gene therapy. BioMed Central 2008-02-12 /pmc/articles/PMC2259350/ /pubmed/18267037 http://dx.doi.org/10.1186/1479-0556-6-6 Text en Copyright © 2008 Redaelli et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Paper
Redaelli, Marco
Cavaggioni, Andrea
Mucignat-Caretta, Carla
Cavirani, Sandro
Caretta, Antonio
Donofrio, Gaetano
Transduction of the rat brain by Bovine Herpesvirus 4
title Transduction of the rat brain by Bovine Herpesvirus 4
title_full Transduction of the rat brain by Bovine Herpesvirus 4
title_fullStr Transduction of the rat brain by Bovine Herpesvirus 4
title_full_unstemmed Transduction of the rat brain by Bovine Herpesvirus 4
title_short Transduction of the rat brain by Bovine Herpesvirus 4
title_sort transduction of the rat brain by bovine herpesvirus 4
topic Short Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2259350/
https://www.ncbi.nlm.nih.gov/pubmed/18267037
http://dx.doi.org/10.1186/1479-0556-6-6
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