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Chronic prehepatic portal hypertension in the rat: is it a type of Metabolic Inflammatory Syndrome?

BACKGROUND: A progressive development of hepatic steatosis with an increase in the lipid hepatocyte content and the formation of megamitochondria have been demonstrated in rats with prehepatic portal hypertension. The aim of this study is to verify the existence of liver and serum lipid metabolism i...

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Autores principales: Sánchez-Patán, Fernando, Anchuelo, Raquel, Aller, Maria-Angeles, Vara, Elena, García, Cruz, Nava, Maria-Paz, Arias, Jaime
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2262079/
https://www.ncbi.nlm.nih.gov/pubmed/18271959
http://dx.doi.org/10.1186/1476-511X-7-4
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author Sánchez-Patán, Fernando
Anchuelo, Raquel
Aller, Maria-Angeles
Vara, Elena
García, Cruz
Nava, Maria-Paz
Arias, Jaime
author_facet Sánchez-Patán, Fernando
Anchuelo, Raquel
Aller, Maria-Angeles
Vara, Elena
García, Cruz
Nava, Maria-Paz
Arias, Jaime
author_sort Sánchez-Patán, Fernando
collection PubMed
description BACKGROUND: A progressive development of hepatic steatosis with an increase in the lipid hepatocyte content and the formation of megamitochondria have been demonstrated in rats with prehepatic portal hypertension. The aim of this study is to verify the existence of liver and serum lipid metabolism impairments in rats with long-term (2 years) portal hypertension. METHODS: Male Wistar rats: Control (n = 10) and with prehepatic portal hypertension by triple partial portal vein ligation (n = 9) were used. Liver content of Triglycerides (TG), phospholipids (PL) and cholesterol and serum cholesterol, lipoproteins (HDL and LDL), TG, glucose and Lipid Binding Protein (LBP) were assayed with specific colorimetric commercial kits. Serum levels of insulin and somatostatin were assayed by RIA. RESULTS: The liver content of TG (6.30 ± 1.95 vs. 4.17 ± 0.59 μg/ml; p < 0.01) and cholesterol (1.48 ± 0.15 vs. 1.10 ± 0.13 μg/ml; p < 0.001) increased in rats with portal hypertension. The serum levels of cholesterol (97.00+26.02 vs. 114.78 ± 37.72 mg/dl), TG (153.41 ± 80.39 vs. 324.39 ± 134.9 mg/dl; p < 0.01), HDL (20.45 ± 5.14 vs. 55.15 ± 17.47 mg/dl; p < 0.001) and somatostatin (1.32 ± 0.31 vs. 1.59 +0.37 mg/dl) decreased, whereas LDL (37.83 ± 15.39 vs. 16.77 ± 6.81 mg/dl; p < 0.001) and LBP (308.47 ± 194.53 vs. 60.27 ± 42.96 ng/ml; p < 0.001) increased. CONCLUSION: Portal hypertension in the rat presents changes in the lipid and carbohydrate metabolisms similar to those produced in chronic inflammatory conditions and sepsis in humans. These underlying alterations could be involved in the development of hepatic steatosis and, therefore, in those described in the metabolic syndrome in humans.
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spelling pubmed-22620792008-03-04 Chronic prehepatic portal hypertension in the rat: is it a type of Metabolic Inflammatory Syndrome? Sánchez-Patán, Fernando Anchuelo, Raquel Aller, Maria-Angeles Vara, Elena García, Cruz Nava, Maria-Paz Arias, Jaime Lipids Health Dis Research BACKGROUND: A progressive development of hepatic steatosis with an increase in the lipid hepatocyte content and the formation of megamitochondria have been demonstrated in rats with prehepatic portal hypertension. The aim of this study is to verify the existence of liver and serum lipid metabolism impairments in rats with long-term (2 years) portal hypertension. METHODS: Male Wistar rats: Control (n = 10) and with prehepatic portal hypertension by triple partial portal vein ligation (n = 9) were used. Liver content of Triglycerides (TG), phospholipids (PL) and cholesterol and serum cholesterol, lipoproteins (HDL and LDL), TG, glucose and Lipid Binding Protein (LBP) were assayed with specific colorimetric commercial kits. Serum levels of insulin and somatostatin were assayed by RIA. RESULTS: The liver content of TG (6.30 ± 1.95 vs. 4.17 ± 0.59 μg/ml; p < 0.01) and cholesterol (1.48 ± 0.15 vs. 1.10 ± 0.13 μg/ml; p < 0.001) increased in rats with portal hypertension. The serum levels of cholesterol (97.00+26.02 vs. 114.78 ± 37.72 mg/dl), TG (153.41 ± 80.39 vs. 324.39 ± 134.9 mg/dl; p < 0.01), HDL (20.45 ± 5.14 vs. 55.15 ± 17.47 mg/dl; p < 0.001) and somatostatin (1.32 ± 0.31 vs. 1.59 +0.37 mg/dl) decreased, whereas LDL (37.83 ± 15.39 vs. 16.77 ± 6.81 mg/dl; p < 0.001) and LBP (308.47 ± 194.53 vs. 60.27 ± 42.96 ng/ml; p < 0.001) increased. CONCLUSION: Portal hypertension in the rat presents changes in the lipid and carbohydrate metabolisms similar to those produced in chronic inflammatory conditions and sepsis in humans. These underlying alterations could be involved in the development of hepatic steatosis and, therefore, in those described in the metabolic syndrome in humans. BioMed Central 2008-02-13 /pmc/articles/PMC2262079/ /pubmed/18271959 http://dx.doi.org/10.1186/1476-511X-7-4 Text en Copyright © 2008 Sánchez-Patán et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Sánchez-Patán, Fernando
Anchuelo, Raquel
Aller, Maria-Angeles
Vara, Elena
García, Cruz
Nava, Maria-Paz
Arias, Jaime
Chronic prehepatic portal hypertension in the rat: is it a type of Metabolic Inflammatory Syndrome?
title Chronic prehepatic portal hypertension in the rat: is it a type of Metabolic Inflammatory Syndrome?
title_full Chronic prehepatic portal hypertension in the rat: is it a type of Metabolic Inflammatory Syndrome?
title_fullStr Chronic prehepatic portal hypertension in the rat: is it a type of Metabolic Inflammatory Syndrome?
title_full_unstemmed Chronic prehepatic portal hypertension in the rat: is it a type of Metabolic Inflammatory Syndrome?
title_short Chronic prehepatic portal hypertension in the rat: is it a type of Metabolic Inflammatory Syndrome?
title_sort chronic prehepatic portal hypertension in the rat: is it a type of metabolic inflammatory syndrome?
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2262079/
https://www.ncbi.nlm.nih.gov/pubmed/18271959
http://dx.doi.org/10.1186/1476-511X-7-4
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