N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging

This project involved the synthesis of N-hexanoyl chitosan or simply modified chitosan (MC) stabilized iron oxide nanoparticles (MC-IOPs) and the biological evaluation of MC-IOPs. IOPs containing MC were prepared using conventional methods, and the extent of cell uptake was evaluated using mouse mac...

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Detalles Bibliográficos
Autores principales: Bhattarai, Shanta R, Kc, Remant B, Kim, Sun Y, Sharma, Manju, Khil, Myung S, Hwang, Pyoung H, Chung, Gyung H, Kim, Hak Y
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265288/
https://www.ncbi.nlm.nih.gov/pubmed/18173857
http://dx.doi.org/10.1186/1477-3155-6-1
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author Bhattarai, Shanta R
Kc, Remant B
Kim, Sun Y
Sharma, Manju
Khil, Myung S
Hwang, Pyoung H
Chung, Gyung H
Kim, Hak Y
author_facet Bhattarai, Shanta R
Kc, Remant B
Kim, Sun Y
Sharma, Manju
Khil, Myung S
Hwang, Pyoung H
Chung, Gyung H
Kim, Hak Y
author_sort Bhattarai, Shanta R
collection PubMed
description This project involved the synthesis of N-hexanoyl chitosan or simply modified chitosan (MC) stabilized iron oxide nanoparticles (MC-IOPs) and the biological evaluation of MC-IOPs. IOPs containing MC were prepared using conventional methods, and the extent of cell uptake was evaluated using mouse macrophages cell line (RAW cells). MC-IOPs were found to rapidly associate with the RAW cells, and saturation was typically reached within the 24 h of incubation at 37°C. Nearly 8.53 ± 0.31 pg iron/cell were bound or internalized at saturation. From these results, we conclude that MC-IOPs effectively deliver into RAW cells in vitro and we also hope MC-IOPs can be used for MRI enhancing agents in biomedical fields.
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spelling pubmed-22652882008-03-07 N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging Bhattarai, Shanta R Kc, Remant B Kim, Sun Y Sharma, Manju Khil, Myung S Hwang, Pyoung H Chung, Gyung H Kim, Hak Y J Nanobiotechnology Research This project involved the synthesis of N-hexanoyl chitosan or simply modified chitosan (MC) stabilized iron oxide nanoparticles (MC-IOPs) and the biological evaluation of MC-IOPs. IOPs containing MC were prepared using conventional methods, and the extent of cell uptake was evaluated using mouse macrophages cell line (RAW cells). MC-IOPs were found to rapidly associate with the RAW cells, and saturation was typically reached within the 24 h of incubation at 37°C. Nearly 8.53 ± 0.31 pg iron/cell were bound or internalized at saturation. From these results, we conclude that MC-IOPs effectively deliver into RAW cells in vitro and we also hope MC-IOPs can be used for MRI enhancing agents in biomedical fields. BioMed Central 2008-01-04 /pmc/articles/PMC2265288/ /pubmed/18173857 http://dx.doi.org/10.1186/1477-3155-6-1 Text en Copyright © 2008 Bhattarai et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Bhattarai, Shanta R
Kc, Remant B
Kim, Sun Y
Sharma, Manju
Khil, Myung S
Hwang, Pyoung H
Chung, Gyung H
Kim, Hak Y
N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging
title N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging
title_full N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging
title_fullStr N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging
title_full_unstemmed N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging
title_short N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging
title_sort n-hexanoyl chitosan stabilized magnetic nanoparticles: implication for cellular labeling and magnetic resonance imaging
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265288/
https://www.ncbi.nlm.nih.gov/pubmed/18173857
http://dx.doi.org/10.1186/1477-3155-6-1
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