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Gene Activation Using FLP Recombinase in C. elegans
The FLP enzyme catalyzes recombination between specific target sequences in DNA. Here we use FLP to temporally and spatially control gene expression in the nematode C. elegans. Transcription is blocked by the presence of an “off cassette” between the promoter and the coding region of the desired pro...
Autores principales: | Davis, M. Wayne, Morton, J. Jason, Carroll, Dana, Jorgensen, Erik M. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265415/ https://www.ncbi.nlm.nih.gov/pubmed/18369447 http://dx.doi.org/10.1371/journal.pgen.1000028 |
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