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RSF Governs Silent Chromatin Formation via Histone H2Av Replacement
Human remodeling and spacing factor (RSF) consists of a heterodimer of Rsf-1 and hSNF2H, a counterpart of Drosophila ISWI. RSF possesses not only chromatin remodeling activity but also chromatin assembly activity in vitro. While no other single factor can execute the same activities as RSF, the biol...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265536/ https://www.ncbi.nlm.nih.gov/pubmed/18454204 http://dx.doi.org/10.1371/journal.pgen.1000011 |
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author | Hanai, Kazuma Furuhashi, Hirofumi Yamamoto, Takashi Akasaka, Koji Hirose, Susumu |
author_facet | Hanai, Kazuma Furuhashi, Hirofumi Yamamoto, Takashi Akasaka, Koji Hirose, Susumu |
author_sort | Hanai, Kazuma |
collection | PubMed |
description | Human remodeling and spacing factor (RSF) consists of a heterodimer of Rsf-1 and hSNF2H, a counterpart of Drosophila ISWI. RSF possesses not only chromatin remodeling activity but also chromatin assembly activity in vitro. While no other single factor can execute the same activities as RSF, the biological significance of RSF remained unknown. To investigate the in vivo function of RSF, we generated a mutant allele of Drosophila Rsf-1 (dRsf-1). The dRsf-1 mutant behaved as a dominant suppressor of position effect variegation. In dRsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin. Furthermore, using both genetic and biochemical approaches, we demonstrate that dRsf-1 interacts with H2Av and the H2Av-exchanging machinery Tip60 complex. These results suggest that RSF contributes to histone H2Av replacement in the pathway of silent chromatin formation. |
format | Text |
id | pubmed-2265536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-22655362008-03-08 RSF Governs Silent Chromatin Formation via Histone H2Av Replacement Hanai, Kazuma Furuhashi, Hirofumi Yamamoto, Takashi Akasaka, Koji Hirose, Susumu PLoS Genet Research Article Human remodeling and spacing factor (RSF) consists of a heterodimer of Rsf-1 and hSNF2H, a counterpart of Drosophila ISWI. RSF possesses not only chromatin remodeling activity but also chromatin assembly activity in vitro. While no other single factor can execute the same activities as RSF, the biological significance of RSF remained unknown. To investigate the in vivo function of RSF, we generated a mutant allele of Drosophila Rsf-1 (dRsf-1). The dRsf-1 mutant behaved as a dominant suppressor of position effect variegation. In dRsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin. Furthermore, using both genetic and biochemical approaches, we demonstrate that dRsf-1 interacts with H2Av and the H2Av-exchanging machinery Tip60 complex. These results suggest that RSF contributes to histone H2Av replacement in the pathway of silent chromatin formation. Public Library of Science 2008-02-29 /pmc/articles/PMC2265536/ /pubmed/18454204 http://dx.doi.org/10.1371/journal.pgen.1000011 Text en Hanai et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hanai, Kazuma Furuhashi, Hirofumi Yamamoto, Takashi Akasaka, Koji Hirose, Susumu RSF Governs Silent Chromatin Formation via Histone H2Av Replacement |
title | RSF Governs Silent Chromatin Formation via Histone H2Av Replacement |
title_full | RSF Governs Silent Chromatin Formation via Histone H2Av Replacement |
title_fullStr | RSF Governs Silent Chromatin Formation via Histone H2Av Replacement |
title_full_unstemmed | RSF Governs Silent Chromatin Formation via Histone H2Av Replacement |
title_short | RSF Governs Silent Chromatin Formation via Histone H2Av Replacement |
title_sort | rsf governs silent chromatin formation via histone h2av replacement |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265536/ https://www.ncbi.nlm.nih.gov/pubmed/18454204 http://dx.doi.org/10.1371/journal.pgen.1000011 |
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