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Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast

Ergosterol depletion independently inhibits two aspects of yeast mating: pheromone signaling and plasma membrane fusion. In signaling, ergosterol participates in the recruitment of Ste5 to a polarized site on the plasma membrane. Ergosterol is thought to form microdomains within the membrane by inte...

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Detalles Bibliográficos
Autores principales: Jin, Hui, McCaffery, J. Michael, Grote, Eric
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265586/
https://www.ncbi.nlm.nih.gov/pubmed/18299351
http://dx.doi.org/10.1083/jcb.200705076
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author Jin, Hui
McCaffery, J. Michael
Grote, Eric
author_facet Jin, Hui
McCaffery, J. Michael
Grote, Eric
author_sort Jin, Hui
collection PubMed
description Ergosterol depletion independently inhibits two aspects of yeast mating: pheromone signaling and plasma membrane fusion. In signaling, ergosterol participates in the recruitment of Ste5 to a polarized site on the plasma membrane. Ergosterol is thought to form microdomains within the membrane by interacting with the long acyl chains of sphingolipids. We find that although sphingolipid-free ergosterol is concentrated at sites of cell–cell contact, transmission of the pheromone signal at contact sites depends on a balanced ratio of ergosterol to sphingolipids. If a mating pair forms between ergosterol-depleted cells despite the attenuated pheromone response, the subsequent process of membrane fusion is retarded. Prm1 also participates in membrane fusion. However, ergosterol and Prm1 have independent functions and only prm1 mutant mating pairs are susceptible to contact-dependent lysis. In contrast to signaling, plasma membrane fusion is relatively insensitive to sphingolipid depletion. Thus, the sphingolipid-free pool of ergosterol promotes plasma membrane fusion.
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spelling pubmed-22655862008-08-25 Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast Jin, Hui McCaffery, J. Michael Grote, Eric J Cell Biol Research Articles Ergosterol depletion independently inhibits two aspects of yeast mating: pheromone signaling and plasma membrane fusion. In signaling, ergosterol participates in the recruitment of Ste5 to a polarized site on the plasma membrane. Ergosterol is thought to form microdomains within the membrane by interacting with the long acyl chains of sphingolipids. We find that although sphingolipid-free ergosterol is concentrated at sites of cell–cell contact, transmission of the pheromone signal at contact sites depends on a balanced ratio of ergosterol to sphingolipids. If a mating pair forms between ergosterol-depleted cells despite the attenuated pheromone response, the subsequent process of membrane fusion is retarded. Prm1 also participates in membrane fusion. However, ergosterol and Prm1 have independent functions and only prm1 mutant mating pairs are susceptible to contact-dependent lysis. In contrast to signaling, plasma membrane fusion is relatively insensitive to sphingolipid depletion. Thus, the sphingolipid-free pool of ergosterol promotes plasma membrane fusion. The Rockefeller University Press 2008-02-25 /pmc/articles/PMC2265586/ /pubmed/18299351 http://dx.doi.org/10.1083/jcb.200705076 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Jin, Hui
McCaffery, J. Michael
Grote, Eric
Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast
title Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast
title_full Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast
title_fullStr Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast
title_full_unstemmed Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast
title_short Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast
title_sort ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265586/
https://www.ncbi.nlm.nih.gov/pubmed/18299351
http://dx.doi.org/10.1083/jcb.200705076
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