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Generation of Functional Fluorescent BK Channels by Random Insertion of GFP Variants
The yellow and cyan variants of green fluorescent protein (GFP) constitute an excellent pair for fluorescence resonance energy transfer (FRET) and can be used to study conformational rearrangements of proteins. Our aim was to develop a library of fluorescent large conductance voltage- and Ca(2+)-gat...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266602/ https://www.ncbi.nlm.nih.gov/pubmed/16260837 http://dx.doi.org/10.1085/jgp.200509368 |