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Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta
BACKGROUND: During natural fertilization, sperm fusion with the oocyte induces long lasting intracellular calcium oscillations which in turn are responsible for oocyte activation. PLCZ1 has been identified as the factor that the sperm delivers into the egg to induce such a response. We tested the hy...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266721/ https://www.ncbi.nlm.nih.gov/pubmed/18284699 http://dx.doi.org/10.1186/1471-213X-8-16 |
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author | Ross, Pablo J Beyhan, Zeki Iager, Amy E Yoon, Sook-Young Malcuit, Christopher Schellander, Karl Fissore, Rafael A Cibelli, Jose B |
author_facet | Ross, Pablo J Beyhan, Zeki Iager, Amy E Yoon, Sook-Young Malcuit, Christopher Schellander, Karl Fissore, Rafael A Cibelli, Jose B |
author_sort | Ross, Pablo J |
collection | PubMed |
description | BACKGROUND: During natural fertilization, sperm fusion with the oocyte induces long lasting intracellular calcium oscillations which in turn are responsible for oocyte activation. PLCZ1 has been identified as the factor that the sperm delivers into the egg to induce such a response. We tested the hypothesis that PLCZ1 cRNA injection can be used to activate bovine oocytes. RESULTS: Mouse and bovine PLCZ1 cRNAs were injected into matured bovine oocytes at different concentrations. Within the concentrations tested, mouse PLCZ1 injection activated bovine oocytes at a maximum rate when the pipette concentration of cRNA ranged from 0.25 to 1 μg/μL, while bovine PLCZ1 was optimal at 0.1 μg/μL. At their most effective concentrations, PLCZ1 induced parthenogenetic development at rates similar to those observed using other activation stimuli such as Ionomycin/CHX and Ionomycin/DMAP. Injection of mouse and bovine PLCZ1 cRNA induced dose-dependent sperm-like calcium oscillations whose frequency increased over time. Injection of bovine and mouse PLCZ1 cRNA also induced IP(3)R-1 degradation, although bovine PLCZ1 cRNA evoked greater receptor degradation than its mouse counterpart. CONCLUSION: Injection of PLCZ1 cRNA efficiently activated bovine oocytes by inducing a sperm-like calcium oscillatory pattern. Importantly, the high rate of aneuploidy encountered in parthenogenetic embryos activated by certain chemical means was not observed in PLCZ1 activated embryos. |
format | Text |
id | pubmed-2266721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22667212008-03-11 Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta Ross, Pablo J Beyhan, Zeki Iager, Amy E Yoon, Sook-Young Malcuit, Christopher Schellander, Karl Fissore, Rafael A Cibelli, Jose B BMC Dev Biol Research Article BACKGROUND: During natural fertilization, sperm fusion with the oocyte induces long lasting intracellular calcium oscillations which in turn are responsible for oocyte activation. PLCZ1 has been identified as the factor that the sperm delivers into the egg to induce such a response. We tested the hypothesis that PLCZ1 cRNA injection can be used to activate bovine oocytes. RESULTS: Mouse and bovine PLCZ1 cRNAs were injected into matured bovine oocytes at different concentrations. Within the concentrations tested, mouse PLCZ1 injection activated bovine oocytes at a maximum rate when the pipette concentration of cRNA ranged from 0.25 to 1 μg/μL, while bovine PLCZ1 was optimal at 0.1 μg/μL. At their most effective concentrations, PLCZ1 induced parthenogenetic development at rates similar to those observed using other activation stimuli such as Ionomycin/CHX and Ionomycin/DMAP. Injection of mouse and bovine PLCZ1 cRNA induced dose-dependent sperm-like calcium oscillations whose frequency increased over time. Injection of bovine and mouse PLCZ1 cRNA also induced IP(3)R-1 degradation, although bovine PLCZ1 cRNA evoked greater receptor degradation than its mouse counterpart. CONCLUSION: Injection of PLCZ1 cRNA efficiently activated bovine oocytes by inducing a sperm-like calcium oscillatory pattern. Importantly, the high rate of aneuploidy encountered in parthenogenetic embryos activated by certain chemical means was not observed in PLCZ1 activated embryos. BioMed Central 2008-02-19 /pmc/articles/PMC2266721/ /pubmed/18284699 http://dx.doi.org/10.1186/1471-213X-8-16 Text en Copyright © 2008 Ross et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ross, Pablo J Beyhan, Zeki Iager, Amy E Yoon, Sook-Young Malcuit, Christopher Schellander, Karl Fissore, Rafael A Cibelli, Jose B Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta |
title | Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta |
title_full | Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta |
title_fullStr | Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta |
title_full_unstemmed | Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta |
title_short | Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta |
title_sort | parthenogenetic activation of bovine oocytes using bovine and murine phospholipase c zeta |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266721/ https://www.ncbi.nlm.nih.gov/pubmed/18284699 http://dx.doi.org/10.1186/1471-213X-8-16 |
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