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Healing Potential of Picrorhiza kurroa (Scrofulariaceae) rhizomes against indomethacin-induced gastric ulceration: a mechanistic exploration.

BACKGROUND: The present study was undertaken to evaluate the potential of the rhizomes of the Indian medicinal plant, Picrorhiza kurroa in healing indomethacin-induced acute stomach ulceration in mice and examine its capacity to modulate oxidative stress and the levels of prostaglandin (PGE(2)) and...

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Autores principales: Banerjee, Debashish, Maity, Biswanath, Nag, Subrata K, Bandyopadhyay, Sandip K, Chattopadhyay, Subrata
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266895/
https://www.ncbi.nlm.nih.gov/pubmed/18237397
http://dx.doi.org/10.1186/1472-6882-8-3
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author Banerjee, Debashish
Maity, Biswanath
Nag, Subrata K
Bandyopadhyay, Sandip K
Chattopadhyay, Subrata
author_facet Banerjee, Debashish
Maity, Biswanath
Nag, Subrata K
Bandyopadhyay, Sandip K
Chattopadhyay, Subrata
author_sort Banerjee, Debashish
collection PubMed
description BACKGROUND: The present study was undertaken to evaluate the potential of the rhizomes of the Indian medicinal plant, Picrorhiza kurroa in healing indomethacin-induced acute stomach ulceration in mice and examine its capacity to modulate oxidative stress and the levels of prostaglandin (PGE(2)) and EGF during the process. METHODS: Male swiss albino mice, ulcerated with indomethacin (18 mg/kg, p. o., single dose) were treated up to 7 days with different doses of the methanol extract of P. kurroa rhizomes (designated as PK). The healing capacity of the most effective dose of PK (20 mg/kg, p. o. × 3 d) was compared with that of omeprazole (Omez) (3 mg/kg, p. o. × 3 d). The effects of the drug-treatment for one and three days on the biochemical parameters were assessed by comparing the results with that of untreated mice of the 1(st )and 3(rd )day of ulceration. The stomach tissues of the mice were used for the biochemical analysis. RESULTS: The macroscopic indices revealed maximum ulceration on the 3(rd )day after indomethacin administration, which was effectively healed by PK. Under the optimized treatment regime, PK and Omez reduced the ulcer indices by 45.1% (P < 0.01), and 76.3% respectively (P < 0.001), compared to the untreated ulcerated mice. Compared to the ulcerated untreated mice, those treated with PK for 3 days showed decreased the levels of thiobarbituric acid reactive substances (TBARS) (32.7%, P < 0.05) and protein carbonyl (37.7%, P < 0.001), and increased mucin (42.2%, P < 0.01), mucosal PGE(2 )(21.4%, P < 0.05), and expressions of COX-1 and 2 (26.9% and 18.5%, P < 0.05), EGF (149.0%, P < 0.001) and VEGF (56.9%, P < 0.01). Omez reduced the TBARS (29.4%, P < 0.05), and protein carbonyl (38.9%, P < 0.001), and increased mucin (38.3%, P < 0.01), without altering the other parameters significantly. CONCLUSION: PK (20 mg/kg, p. o. × 3 days) could effectively heal indomethacin-induced stomach ulceration in mice by reducing oxidative stress, and promoting mucin secretion, prostaglandin synthesis and augmenting expressions of cyclooxygenase enzymes and growth factors.
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spelling pubmed-22668952008-03-12 Healing Potential of Picrorhiza kurroa (Scrofulariaceae) rhizomes against indomethacin-induced gastric ulceration: a mechanistic exploration. Banerjee, Debashish Maity, Biswanath Nag, Subrata K Bandyopadhyay, Sandip K Chattopadhyay, Subrata BMC Complement Altern Med Research Article BACKGROUND: The present study was undertaken to evaluate the potential of the rhizomes of the Indian medicinal plant, Picrorhiza kurroa in healing indomethacin-induced acute stomach ulceration in mice and examine its capacity to modulate oxidative stress and the levels of prostaglandin (PGE(2)) and EGF during the process. METHODS: Male swiss albino mice, ulcerated with indomethacin (18 mg/kg, p. o., single dose) were treated up to 7 days with different doses of the methanol extract of P. kurroa rhizomes (designated as PK). The healing capacity of the most effective dose of PK (20 mg/kg, p. o. × 3 d) was compared with that of omeprazole (Omez) (3 mg/kg, p. o. × 3 d). The effects of the drug-treatment for one and three days on the biochemical parameters were assessed by comparing the results with that of untreated mice of the 1(st )and 3(rd )day of ulceration. The stomach tissues of the mice were used for the biochemical analysis. RESULTS: The macroscopic indices revealed maximum ulceration on the 3(rd )day after indomethacin administration, which was effectively healed by PK. Under the optimized treatment regime, PK and Omez reduced the ulcer indices by 45.1% (P < 0.01), and 76.3% respectively (P < 0.001), compared to the untreated ulcerated mice. Compared to the ulcerated untreated mice, those treated with PK for 3 days showed decreased the levels of thiobarbituric acid reactive substances (TBARS) (32.7%, P < 0.05) and protein carbonyl (37.7%, P < 0.001), and increased mucin (42.2%, P < 0.01), mucosal PGE(2 )(21.4%, P < 0.05), and expressions of COX-1 and 2 (26.9% and 18.5%, P < 0.05), EGF (149.0%, P < 0.001) and VEGF (56.9%, P < 0.01). Omez reduced the TBARS (29.4%, P < 0.05), and protein carbonyl (38.9%, P < 0.001), and increased mucin (38.3%, P < 0.01), without altering the other parameters significantly. CONCLUSION: PK (20 mg/kg, p. o. × 3 days) could effectively heal indomethacin-induced stomach ulceration in mice by reducing oxidative stress, and promoting mucin secretion, prostaglandin synthesis and augmenting expressions of cyclooxygenase enzymes and growth factors. BioMed Central 2008-01-31 /pmc/articles/PMC2266895/ /pubmed/18237397 http://dx.doi.org/10.1186/1472-6882-8-3 Text en Copyright © 2008 Banerjee et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Banerjee, Debashish
Maity, Biswanath
Nag, Subrata K
Bandyopadhyay, Sandip K
Chattopadhyay, Subrata
Healing Potential of Picrorhiza kurroa (Scrofulariaceae) rhizomes against indomethacin-induced gastric ulceration: a mechanistic exploration.
title Healing Potential of Picrorhiza kurroa (Scrofulariaceae) rhizomes against indomethacin-induced gastric ulceration: a mechanistic exploration.
title_full Healing Potential of Picrorhiza kurroa (Scrofulariaceae) rhizomes against indomethacin-induced gastric ulceration: a mechanistic exploration.
title_fullStr Healing Potential of Picrorhiza kurroa (Scrofulariaceae) rhizomes against indomethacin-induced gastric ulceration: a mechanistic exploration.
title_full_unstemmed Healing Potential of Picrorhiza kurroa (Scrofulariaceae) rhizomes against indomethacin-induced gastric ulceration: a mechanistic exploration.
title_short Healing Potential of Picrorhiza kurroa (Scrofulariaceae) rhizomes against indomethacin-induced gastric ulceration: a mechanistic exploration.
title_sort healing potential of picrorhiza kurroa (scrofulariaceae) rhizomes against indomethacin-induced gastric ulceration: a mechanistic exploration.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266895/
https://www.ncbi.nlm.nih.gov/pubmed/18237397
http://dx.doi.org/10.1186/1472-6882-8-3
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