Genetically delivered antibody protects against West Nile virus

Gene-based delivery of recombinant antibody genes is a promising therapeutic strategy offering numerous advantages including sustained antibody levels, better safety profile and lower production cost. Here we describe generation of a recombinant antibody Fc-9E2 comprising a fusion protein between hu...

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Autores principales: Pereboev, Alexander, Borisevich, Viktoriya, Tsuladze, George, Shakhmatov, Mikhail, Hudman, Deborah, Kazachinskaia, Elena, Razumov, Ivan, Svyatchenko, Viktor, Loktev, Valery, Yamshchikov, Vladimir
Formato: Texto
Lenguaje:English
Publicado: Elsevier B.V. 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2267767/
https://www.ncbi.nlm.nih.gov/pubmed/17904654
http://dx.doi.org/10.1016/j.antiviral.2007.08.010
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author Pereboev, Alexander
Borisevich, Viktoriya
Tsuladze, George
Shakhmatov, Mikhail
Hudman, Deborah
Kazachinskaia, Elena
Razumov, Ivan
Svyatchenko, Viktor
Loktev, Valery
Yamshchikov, Vladimir
author_facet Pereboev, Alexander
Borisevich, Viktoriya
Tsuladze, George
Shakhmatov, Mikhail
Hudman, Deborah
Kazachinskaia, Elena
Razumov, Ivan
Svyatchenko, Viktor
Loktev, Valery
Yamshchikov, Vladimir
author_sort Pereboev, Alexander
collection PubMed
description Gene-based delivery of recombinant antibody genes is a promising therapeutic strategy offering numerous advantages including sustained antibody levels, better safety profile and lower production cost. Here we describe generation of a recombinant antibody Fc-9E2 comprising a fusion protein between human Fc of IgG1 and a single-chain Fv derived from a hybridoma 9E2 secreting a mAb neutralizing West Nile virus (WNV). Fc-9E2 was shown to retain parental mAb's specificity and WNV-neutralizing capacity. Adenovirus-mediated in vivo delivery of the antibody gene resulted in sustained Fc-9E2 serum levels leading to abrogation of lethal WNV infection in an animal model.
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spelling pubmed-22677672008-03-14 Genetically delivered antibody protects against West Nile virus Pereboev, Alexander Borisevich, Viktoriya Tsuladze, George Shakhmatov, Mikhail Hudman, Deborah Kazachinskaia, Elena Razumov, Ivan Svyatchenko, Viktor Loktev, Valery Yamshchikov, Vladimir Antiviral Res Article Gene-based delivery of recombinant antibody genes is a promising therapeutic strategy offering numerous advantages including sustained antibody levels, better safety profile and lower production cost. Here we describe generation of a recombinant antibody Fc-9E2 comprising a fusion protein between human Fc of IgG1 and a single-chain Fv derived from a hybridoma 9E2 secreting a mAb neutralizing West Nile virus (WNV). Fc-9E2 was shown to retain parental mAb's specificity and WNV-neutralizing capacity. Adenovirus-mediated in vivo delivery of the antibody gene resulted in sustained Fc-9E2 serum levels leading to abrogation of lethal WNV infection in an animal model. Elsevier B.V. 2008-01 2007-09-14 /pmc/articles/PMC2267767/ /pubmed/17904654 http://dx.doi.org/10.1016/j.antiviral.2007.08.010 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Pereboev, Alexander
Borisevich, Viktoriya
Tsuladze, George
Shakhmatov, Mikhail
Hudman, Deborah
Kazachinskaia, Elena
Razumov, Ivan
Svyatchenko, Viktor
Loktev, Valery
Yamshchikov, Vladimir
Genetically delivered antibody protects against West Nile virus
title Genetically delivered antibody protects against West Nile virus
title_full Genetically delivered antibody protects against West Nile virus
title_fullStr Genetically delivered antibody protects against West Nile virus
title_full_unstemmed Genetically delivered antibody protects against West Nile virus
title_short Genetically delivered antibody protects against West Nile virus
title_sort genetically delivered antibody protects against west nile virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2267767/
https://www.ncbi.nlm.nih.gov/pubmed/17904654
http://dx.doi.org/10.1016/j.antiviral.2007.08.010
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