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Comparative analysis of the tear protein profile in mycotic keratitis patients
PURPOSE: Mycotic keratitis is a major cause of corneal blindness in India. A proper understanding of the pathogenesis may help in refining the existing treatment. The purpose of this study is to examine the total tear protein profile of fungal keratitis patients, which may have a bearing on pathogen...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Molecular Vision
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268856/ https://www.ncbi.nlm.nih.gov/pubmed/18385783 |
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author | Ananthi, Sivagnanam Chitra, Thangavel Bini, Ramachandran Prajna, Namperumalsamy Venkatesh Lalitha, Prajna Dharmalingam, Kuppamuthu |
author_facet | Ananthi, Sivagnanam Chitra, Thangavel Bini, Ramachandran Prajna, Namperumalsamy Venkatesh Lalitha, Prajna Dharmalingam, Kuppamuthu |
author_sort | Ananthi, Sivagnanam |
collection | PubMed |
description | PURPOSE: Mycotic keratitis is a major cause of corneal blindness in India. A proper understanding of the pathogenesis may help in refining the existing treatment. The purpose of this study is to examine the total tear protein profile of fungal keratitis patients, which may have a bearing on pathogenesis and disease progression. METHODS: Tear samples were collected from culture positive fungal keratitis patients. Tears from the uninfected fellow eye and from other healthy individuals served as controls. Two-dimensional electrophoresis (2DE) was used for the separation of fractionated tear proteins, and selected protein spots, which showed differential expressions, were identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Wherever needed, tag sequencing of peptide fragments using post source decay (PSD) was done to confirm the identification. RESULTS: The glutaredoxin-related protein was expressed only in the tears of fungal keratitis patients. Six other normal tear proteins were present in both samples but with varied expression levels. Prolactin inducible protein and serum albumin precursor were upregulated in the infected samples. Cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin were downregulated in the infected samples. CONCLUSIONS: Tears can be used as a clinical source to study the proteomic responses in patients with fungal keratitis. The glutaredoxin-related protein is known to be produced by Aspergillus during oxidative stress conditions, and the presence of this protein in the tears of patients with mycotic keratitis indicates that this pathogen undergoes stress-related gene expression during infection. |
format | Text |
id | pubmed-2268856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-22688562008-03-20 Comparative analysis of the tear protein profile in mycotic keratitis patients Ananthi, Sivagnanam Chitra, Thangavel Bini, Ramachandran Prajna, Namperumalsamy Venkatesh Lalitha, Prajna Dharmalingam, Kuppamuthu Mol Vis Research Article PURPOSE: Mycotic keratitis is a major cause of corneal blindness in India. A proper understanding of the pathogenesis may help in refining the existing treatment. The purpose of this study is to examine the total tear protein profile of fungal keratitis patients, which may have a bearing on pathogenesis and disease progression. METHODS: Tear samples were collected from culture positive fungal keratitis patients. Tears from the uninfected fellow eye and from other healthy individuals served as controls. Two-dimensional electrophoresis (2DE) was used for the separation of fractionated tear proteins, and selected protein spots, which showed differential expressions, were identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Wherever needed, tag sequencing of peptide fragments using post source decay (PSD) was done to confirm the identification. RESULTS: The glutaredoxin-related protein was expressed only in the tears of fungal keratitis patients. Six other normal tear proteins were present in both samples but with varied expression levels. Prolactin inducible protein and serum albumin precursor were upregulated in the infected samples. Cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin were downregulated in the infected samples. CONCLUSIONS: Tears can be used as a clinical source to study the proteomic responses in patients with fungal keratitis. The glutaredoxin-related protein is known to be produced by Aspergillus during oxidative stress conditions, and the presence of this protein in the tears of patients with mycotic keratitis indicates that this pathogen undergoes stress-related gene expression during infection. Molecular Vision 2008-03-12 /pmc/articles/PMC2268856/ /pubmed/18385783 Text en http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ananthi, Sivagnanam Chitra, Thangavel Bini, Ramachandran Prajna, Namperumalsamy Venkatesh Lalitha, Prajna Dharmalingam, Kuppamuthu Comparative analysis of the tear protein profile in mycotic keratitis patients |
title | Comparative analysis of the tear protein profile in mycotic keratitis patients |
title_full | Comparative analysis of the tear protein profile in mycotic keratitis patients |
title_fullStr | Comparative analysis of the tear protein profile in mycotic keratitis patients |
title_full_unstemmed | Comparative analysis of the tear protein profile in mycotic keratitis patients |
title_short | Comparative analysis of the tear protein profile in mycotic keratitis patients |
title_sort | comparative analysis of the tear protein profile in mycotic keratitis patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268856/ https://www.ncbi.nlm.nih.gov/pubmed/18385783 |
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